cat no | io1114
ioGlutamatergic Neurons APOE 4/4
Human iPSC-derived Alzheimer's disease risk model
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Cryopreserved human iPSC-derived cells powered by opti-ox that are ready for experiments in days
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In vitro cell model engineered with an APOE 4/4 genotype for Alzheimer's disease research
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Consistent, functional excitatory neurons that form neuronal networks within days
Human iPSC-derived neuronal APOE 4/4 model
Expression of DAPI and MAP2 in glutamatergic neurons with APOE 4/4 genotype.
ioGlutamatergic Neurons Media Kit
Cell culture media kit for the culture of ioGlutamatergic Neurons up to 14 days post-thaw
ioGlutamatergic Neurons APOE 4/4 express neuron-specific markers comparably to the wild type control
Immunofluorescent staining on day 11 post-revival demonstrates similar homogeneous expression of pan-neuronal proteins MAP2 and TUBB3 (upper panel) and glutamatergic neuron-specific transporter VGLUT2 (lower panel) in ioGlutamatergic Neurons APOE 4/4 compared to the genetically matched control (10x objective).
ioGlutamatergic Neurons APOE 4/4 form structural neuronal networks by day 11
ioGlutamatergic Neurons APOE 4/4 mature rapidly, show glutamatergic neuron morphology and form structural neuronal networks over 11 days, highly similar to the genetically matched control. Day 1 to 11 post thaw; (10x objective).
ioGlutamatergic Neurons APOE 4/4 demonstrate gene expression of neuronal-specific and glutamatergic-specific markers following deterministic cell programming
Gene expression analysis demonstrates that ioGlutamatergic Neurons APOE 4/4 and wild type ioGlutamatergic Neurons (WT Control) lack the expression of pluripotency marker POU5F1 (OCT4) at day 11, while robustly expressing pan-neuronal (TUBB3 and SYP) and glutamatergic-specific (VGLUT1 and VGLUT2) markers. Gene expression levels were assessed by RT-qPCR (data normalised to HMBS; cDNA samples of the parental human iPSC line (hiPSC) were included as reference). Data represents day 11 post-revival samples, n=2 replicates.
View the step-by-step RNA extraction and RT-qPCR protocol used to generate this data
Phenotypic characterisation of a human iPSC-derived tri-culture using ioGlutamatergic Neurons, ioAstrocytes, and ioMicroglia
Using our fully optimised protocol, ioGlutamatergic Neurons (MAP2, red), ioMicroglia (IBA1, yellow) and ioAstrocytes (vimentin, cyan) were co-cultured to create a highly defined CNS model. High-resolution ICC analysis confirms the successful co-localisation and morphological health of three distinct cell types within a unified environment. By day 7, the protocol yields a highly consistent, integrated network suitable for complex cell modelling. DAPI (blue) highlights the total cell density and integrity of the culture. This protocol is compatible with derivative products of the three cell types, ensuring straightforward implementation across experimental workflows.
Efficient mRNA transfection into ioGlutamatergic Neurons
ioGlutamatergic Neurons are efficiently transfected and show sustained long-term expression of mRNA encoding GFP. ioGlutamatergic Neurons were imaged from day 1 post-thaw and throughout the experiment to assess transfection efficiency and evaluate potential cytotoxic effects of the transfection protocol. Day 1 images were captured prior to transfection on the same day.
Download the step-by-step protocol for lipid-based delivery of synthetic mRNA into ioGlutamatergic Neurons.
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