cat no | io1001
ioGlutamatergic Neurons from human induced pluripotent stem cells (iPSC) deterministically programmed using opti-ox technology. Within days, cells convert consistently to mature, functional glutamatergic neurons characterised by >80% expression of glutamate transporter genes VGLUT1 and VGLUT2.
Glutamatergic neurons are delivered cryopreserved and ready-to-culture making them a high-quality human model for fundamental research, disease modelling and drug discovery.
Confidently investigate your phenotype of interest across multiple clones with our disease model clone panel. Detailed characterisation data (below) and bulk RNA sequencing data (upon request) help you select specific clones if required.
per vial
A maximum number of 20 vials applies. If you would like to order more than 20 vials, please contact us at orders@bit.bio.
Quick
Ready for experimentation as early as 2 days post revival and form functional neuronal networks at 17 days.
Scalable
Industrial scale quantities at a price point that allows the cells to be used from research to screening scale.
Easy to use
Cells arrive programmed to rapidly mature upon revival. One medium is required in a two-step protocol.
ioGlutamatergic Neurons generated by transcription factor-driven deterministic programming of iPSCs using opti-ox technology
ioGlutamatergic Neurons express glutamatergic neuron-specific markers
ioGlutamatergic Neurons form structural neuronal networks by day 11
Whole transcriptome analysis demonstrates high lot-to-lot consistency across three manufactured lots of ioGlutamatergic Neurons
High lot-to-lot consistency is demonstrated by a consistent transcriptomic fingerprint across manufactured lots of ioGlutamatergic Neurons
Single cell ATAC-sequencing shows a consistent transcriptomic fingerprint demonstrating high lot-to-lot consistency across manufactured lots of ioGlutamatergic Neurons
ioGlutamatergic Neurons show good suitability for high-throughput screening in 384-well format plates
Do more with every vial
Cells arrive ready to plate
bit.bio
V11
bit.bio
2024
Professor Deepak Srivastava
Professor of Molecular Neuroscience and Group Leader, MRC Centre for Developmental Disorders
King’s College London
Read this blog on glutamatergic neuron cell culture for our top tips on careful handling, cell plating and media changes to achieve success from the outset.
Dr Shushant Jain
Group Leader | In Vitro Biology | Charles River, 2021
Dr Mariangela Iovino
Senior Group Leader | Biology Discovery | Charles River
Dr Koby Baranes
Research Associate | University of Cambridge
Dr Jeremy Anton
Scientist | Charles River
Dr Deepak Srivastava
Reader in Molecular Neuroscience | King's College London
Be confident in your data by pairing ioDisease Model cells with the genetically matched ioWild Type control