Immunocytochemistry staining for ioMicroglia

Immunocytochemistry (ICC) staining is a powerful tool that enables specific proteins or other molecules to be quantitatively visualised in a cellular context. As such, ICC can provide insights into the distribution, localisation, and abundance of specific proteins or other molecules. While ICC staining of iPSC-derived cells is a relatively straightforward process, it does require careful consideration of antibody pairings, delicate treatment of cells, and optimisation to reduce non-specific signals.

The protocol outlines recommended reagents and concentrations for the fixation, permeabilisation, and antibody staining of key markers relevant to microglia. While these specific antibodies and dilutions are recommended for reliable outcomes, the core protocol can be adapted by researchers for use with other primary antibodies of interest.