User manual

ioAstrocytes

cat no ioEA1093 | Human iPSC-derived astrocytes
ioAstrocytes
cat no ioEA1093 | Human iPSC-derived astrocytes

ioAstrocytes are delivered in a cryopreserved format. The user manual for the generation of three cells follows a simple three-phase process.

  • Induction
    Human iPSCs are exposed to an induction protocol. This leads to irreversible loss of pluripotency, and the synchronised homogeneous production of ioAstrocytes. The ioAstrocytes are subsequently cryopreserved for distribution.
  • Phase 1 – Stabilisation (day 0 to day 2)
    The ioAstrocytes are revived at the user’s laboratory using the recommended medium. After 24h a full media change.
  • Phase 2 – Pre-maintenance (day 2 to day 8)
    Every 48h a 50% media change is performed with the recommended medium.
  • Phase 3 – Maintenance (day 8 onwards):
    The ioAstrocytes require full media change switching to recommended medium. Half media changes are subsequently performed every 48h onwards.

V6

bit.bio

2024

Related pages

News Read our latest updates and press coverage
Our platform Discover the cell coding platform behind our cells
Join us Explore the latest roles at bit.bio