Human iPSC-derived skeletal muscle cells

Consistent, defined and scalable human iPSC-derived skeletal myocytes for musculoskeletal disorders (MSD) research

Significant advances are being made in the study of musculoskeletal disease. Where conditions like Duchenne muscular dystrophy (DMD) were once seen as untreatable, hope now swells as gene therapies advance into the market. Critical to the study and treatment of conditions like DMD is the use of physiologically relevant musculoskeletal disease models. As immortalised human muscle cell lines can be difficult to access, and animal myoblast cell lines are phenotypically distinct from their in vivo counterparts, many in the field are now turning to a more physiologically accurate source of cells: iPSC-derived myocytes [1].

bit.bio’s deterministic cell programming technology (known as opti-ox™) makes it possible to generate functional human skeletal muscle cells with unmatched lot-to-lot consistency from iPSCs on a nearly limitless scale (all without the challenges typically associated with myocyte differentiation). Both wild-type and disease-specific myocytes are readily available, enabling researchers to study skeletal myocytes in vitro with ever more translational power.

Leveraging genetically matched controls, human iPSC-derived skeletal myocytes containing DMD mutations can be used to screen prospective therapeutics, including exon-skipping therapies and other advanced drug modalities. 

Strengthen your research with bit.bio’s human iPSC-derived skeletal myocytes.

Engineered to meet your workflow with our toolkit of ioWild Type Cells and ioDisease Model Cells

ioWild Type Cells

ioSkeletal Myocytes cat no | io1002

ioDisease Model Cells

ioSkeletal Myocytes DMD Exon 44 Deletion cat no | io1018

ioDisease Model Cells

ioSkeletal Myocytes DMD Exon 52 Deletion cat no | io1019

Driving a better understanding of musculoskeletal disease

Dr Rita Horvath, Director of Research in Genetics of Rare Neurological Disorders at the University of Cambridge discusses the mechanical and metabolic roles of myocytes in health and disease, experimental limitations and new opportunities offered by consistent, scalable human myocytes for muscle research and disease modelling.

Read blog

Browse relevant resources

(LinkedValues: [{hs_name=ioSkeletal Myocytes , hs_id=161746296924, hs_path=skeletal-myocytes, button_label=View brochure, button_link=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Resources/Brochures/bit.bio-ioSkeletal%20Myocytes%20Brochure.pdf, type={label=Brochure, value=Brochure}, thumbnail={alt_text=, width=2550, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Product%20pages/ioSkeletal%20Myocytes/ioSkeletal-Myocytes-2550px_wide-banner-comp.jpeg, height=1913}, year={label=2024, value=2024}, summary=<p>bit.bio</p>, date_published=1713312000000, sort_date=1713312000000, tags=[{label=ioSkeletal Myocytes, value=ioSkeletal Myocytes}, {label=ioSkeletal Myocytes DMD44, value=ioSkeletal Myocytes DMD44}, {label=ioSkeletal Myocytes DMD52, value=ioSkeletal Myocytes DMD52}, {label=Product information, value=Product information}], media_contact=null, listing_button_label=Download}, {hs_name=Genotype and phenotype validation of an isogenic human iPSC-derived neuronal model of Huntington’s Disease, hs_id=161961134490, hs_path=huntingtons-htt50cag-phenotype-poster-mar23, button_label=View poster, button_link=https://drive.google.com/file/d/1BxJNslvtuCrc2G10C0SqwbfYx3h7THNk/view, type={label=Poster, value=Poster}, thumbnail={alt_text=, width=600, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Product%20pages/ioGlutamatergic%20Neurons%20HTT%2050CAGWT/a-HTT50CAGWT_Overlay__TUBB3(G)_MAP2(R)_DAPI(B)_%20(1).png, height=378}, year={label=2022, value=2022}, summary=<p>Oosterveen, et al</p> <p>bit.bio</p> <p>2022</p>, date_published=1708819200000, sort_date=1679443200000, tags=[{label=ioSkeletal Myocytes, value=ioSkeletal Myocytes}, {label=ioGlutamatergic Neurons TDP-43 M337V Het, value=ioGlutamatergic Neurons TDP-43 M337V Het}, {label=ioGlutamatergic Neurons TDP-43 M337V Hom, value=ioGlutamatergic Neurons TDP-43 M337V Hom}, {label=ioGlutamatergic Neurons MAPT P301S/WT, value=ioGlutamatergic Neurons MAPT P301S/WT}, {label=ioGlutamatergic Neurons MAPT P301S/P301S, value=ioGlutamatergic Neurons MAPT P301S/P301S}, {label=ioGlutamatergic Neurons MAPT N279K/WT, value=ioGlutamatergic Neurons MAPT N279K/WT}], media_contact=null, listing_button_label=View}, {hs_name=Generation of 3D skeletal muscle microtissues using ioSkeletal Myocytes, hs_id=161961134493, hs_path=generation-of-3d-skeletal-muscle-microtissues-using-ioskeletal-myocytes, button_label=View poster, button_link=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Product%20collateral/Posters/Stimulating%203D%20Skeletal%20Muscle%20Microtissues%20in%20Perfusable%20Microphysiological%20System_Biond_Poster_MPS_Berlin_Jun23.pdf, type={label=Poster, value=Poster}, thumbnail={alt_text=, width=1932, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/horizontal_ioSkeletal-Myocytes-Muscle-Bundle-Biond-ICC-SAA-Actin%20(1)-1%20(1).png, height=1440}, year={label=2023, value=2023}, summary=<p>Dr Mitchell Han</p> <p>Bi/ond</p> <p>2023</p>, date_published=1710028800000, sort_date=1693267200000, tags=[{label=ioSkeletal Myocytes, value=ioSkeletal Myocytes}, {label=ioSkeletal Myocytes DMD44, value=ioSkeletal Myocytes DMD44}, {label=ioSkeletal Myocytes DMD52, value=ioSkeletal Myocytes DMD52}], media_contact=null, listing_button_label=View }, {hs_name=Human iPSC-derived DMD skeletal myocytes for 3D functional studies and dystrophin restoration, hs_id=173835555607, hs_path=isscr2024-skeletal-myocytes-dmd-3d-function-dystrophin-restoration, button_label=null, button_link=14527135.fs1.hubspotusercontent-na1.net, type={label=Poster, value=Poster}, thumbnail={alt_text=ISSCR24-DMD-Exon44-3D-muscle-bundle (1), width=2953, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/ISSCR24-DMD-Exon44-3D-muscle-bundle%20(1).png, height=1969}, year={label=2024, value=2024}, summary=<p>Bernard, et al</p> <p>bit.bio</p> <p>2024</p>, date_published=1721692800000, sort_date=1721692800000, tags=[{label=ioSkeletal Myocytes, value=ioSkeletal Myocytes}, {label=ioSkeletal Myocytes DMD44, value=ioSkeletal Myocytes DMD44}, {label=ioSkeletal Myocytes DMD52, value=ioSkeletal Myocytes DMD52}], media_contact=<p>Bernard et al,</p> <p>bit.bio</p> <p>2024</p>, listing_button_label=Download}, {hs_name=Reprogramming the stem cell for a new generation of cures, hs_id=161968263465, hs_path=reprogramming-the-stem-cell-for-a-new-generation-of-cures, button_label=Read more, button_link=https://www.ddw-online.com/reprogramming-the-stem-cell-for-a-new-generation-of-cures-1459-202004/, type={label=Publication, value=Publication}, thumbnail={alt_text=, width=2092, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/bit.bio%20ioGlutamatergic%20Neurons%202022.jpg, height=1584}, year={label=2020, value=2020}, summary=<p>Davenport A, Frolov T &amp; Kotter M</p> <p><em>Drug Discovery World</em></p> <p>2020</p> <p>&nbsp;</p> <p>&nbsp;</p>, date_published=1585872000000, sort_date=1597968000000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioSkeletal Myocytes, value=ioSkeletal Myocytes}, {label=ioGABAergic Neurons, value=ioGABAergic Neurons}, {label=ioMicroglia, value=ioMicroglia}, {label=ioSensory Neurons, value=ioSensory Neurons}, {label=ioMotor Neurons, value=ioMotor Neurons}, {label=ioAstrocytes, value=ioAstrocytes}], media_contact=<p><span>Davenport A, Frolov T &amp; Kotter M.</span></p> <p><em>Drug Discovery World</em></p> <p>2020</p> <p>&nbsp;</p> <p>&nbsp;</p>, listing_button_label=Read more}, {hs_name=Functional neurological restoration of amputated peripheral nerve using biohybrid regenerative bioelectronics | Publication, hs_id=161968263470, hs_path=restoration-of-nerve-using-biohybrid-regenerative-bioelectronics, button_label=Read more, button_link=https://www.science.org/doi/10.1126/sciadv.add8162#, type={label=Publication, value=Publication}, thumbnail={alt_text=, width=2550, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/News%20images/biohybrid%20resized.jpg, height=1343}, year={label=2023, value=2023}, summary=<p>Rochford and Carnicer-Lombarte et al.</p> <p><em>Science Advances</em></p> <p>2023</p> <p>Featuring <a href="https://www.google.com/url?q=https://www.bit.bio/platform&amp;source=gmail-imap&amp;ust=1727249746000000&amp;usg=AOvVaw1Bye_dZCELecMlJxS10ZnH" rel="noopener">opti-ox </a>enabled skeletal myocytes iPS cell line</p>, date_published=1679443200000, sort_date=1680652800000, tags=[{label=ioSkeletal Myocytes, value=ioSkeletal Myocytes}, {label=ioSkeletal Myocytes DMD44, value=ioSkeletal Myocytes DMD44}, {label=ioSkeletal Myocytes DMD52, value=ioSkeletal Myocytes DMD52}], media_contact=<p>Rochford and Carnicer-Lombarte et al.</p> <p><em>Science Advances</em></p> <p>2023</p> <p>Featuring <a href="https://www.google.com/url?q=https://www.bit.bio/platform&amp;source=gmail-imap&amp;ust=1727249746000000&amp;usg=AOvVaw1Bye_dZCELecMlJxS10ZnH" rel="noopener">opti-ox</a> enabled skeletal myocytes iPS cell line</p>, listing_button_label=Read more}, {hs_name=CRISPR and the Art of Perturbation Screening: Unbiased functional genomic screening meets the best human cellular models, hs_id=161968263475, hs_path=crispr-and-the-art-of-perturbation-screening-unbiased-functional-genomic-screening-meets-the-best-human-cellular-models, button_label=Explore ioCRISPR-Ready Cells, button_link=https://www.bit.bio/iocrispr-ready, type={label=Talk, value=Talk}, thumbnail={alt_text=, width=1318, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/BitBio2022/Resources%20listing/hubdb-thumbs/resources/CRISPR%20and%20the%20Art%20of%20Perturbation%20Screening-min.png, height=1000}, year={label=2021, value=2021}, summary=<p>Kam Dhaliwal <span>| </span>SVP Strategic Alliances | bit.bio</p> <p><br>Talk at ELRIG CRISPR in Drug Discovery</p>, date_published=1706140800000, sort_date=1636070400000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioSkeletal Myocytes, value=ioSkeletal Myocytes}, {label=ioGlutamatergic Neurons HTT 50CAG/WT, value=ioGlutamatergic Neurons HTT 50CAG/WT}, {label=ioSensory Neurons, value=ioSensory Neurons}], media_contact=null, listing_button_label=Watch now}, {hs_name=Consistent and scalable human iPSC-derived cells for in vitro disease modelling and drug discovery, hs_id=161968263476, hs_path=consistent-and-scalable-human-ipsc-derived-cells-for-in-vitro-disease-modelling-and-drug-discovery, button_label=Explore ioCells, button_link=https://www.bit.bio/discover-iocells, type={label=Talk, value=Talk}, thumbnail={alt_text=, width=900, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/DDD_banner_square.jpeg, height=652}, year={label=2020, value=2020}, summary=<p>Kam Dhaliwal <span>|&nbsp;</span> SVP Strategic Alliances | bit.bio<br>Dr Thomas Moreau <span>|</span> Head of Research | bit.bio</p> <p><br>Talk at ELRIG Drug Discovery Digital</p>, date_published=1706400000000, sort_date=1600905600000, tags=[{label=ioGlutamatergic Neurons, value=ioGlutamatergic Neurons}, {label=ioSkeletal Myocytes, value=ioSkeletal Myocytes}, {label=ioGlutamatergic Neurons HTT 50CAG/WT, value=ioGlutamatergic Neurons HTT 50CAG/WT}], media_contact=null, listing_button_label=Watch now}, {hs_name=ioSkeletal Myocytes and related disease models | User Manual, hs_id=161968263488, hs_path=skeletal-myocytes, button_label=null, button_link=null, type={label=User manual, value=User manual}, thumbnail={alt_text=, width=2550, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Product%20pages/ioSkeletal%20Myocytes/ioSkeletal-Myocytes-2550px_wide-banner-comp.jpeg, height=1913}, year={label=2023, value=2023}, summary=<p><span>V5</span></p> <p><span>bit.bio</span></p> <p><span>2023</span></p>, date_published=1708041600000, sort_date=1708041600000, tags=[{label=ioSkeletal Myocytes, value=ioSkeletal Myocytes}, {label=Product information, value=Product information}], media_contact=<p><span>V5</span></p> <p><span>bit.bio</span></p> <p><span>2023</span></p>, listing_button_label=Download}, {hs_name=Introducing ioSkeletal Myocytes | Developing the next generation of human muscle cells, hs_id=161968263500, hs_path=introducing-ioskeletal-myocytes, button_label=null, button_link=null, type={label=Video, value=Video}, thumbnail={alt_text=, width=1318, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/BitBio2022/product-page/Introducing%20ioSkeletal%20Myocytes-min-comp.jpg, height=1000}, year={label=2022, value=2022}, summary=<p><span>Dr Will Bernard | Director of Cell Type Development | </span><span>bit.bio</span></p>, date_published=1707264000000, sort_date=1643068800000, tags=[{label=ioSkeletal Myocytes, value=ioSkeletal Myocytes}, {label=Product information, value=Product information}, {label=ioSkeletal Myocytes DMD44, value=ioSkeletal Myocytes DMD44}, {label=ioSkeletal Myocytes DMD52, value=ioSkeletal Myocytes DMD52}], media_contact=null, listing_button_label=Watch}, {hs_name=Advances in cellular reprogramming: from stem cells to printed tissues, hs_id=161968263517, hs_path=advances-in-cellular-reprogramming-from-stem-cells-to-printed-tissues, button_label=Explore ioCells, button_link=https://www.bit.bio/discover-iocells, type={label=Webinar, value=Webinar}, thumbnail={alt_text=, width=666, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/BitBio2022/Resources/Resource-thumbs/resized/New%20Project%20(13).jpeg, height=428}, year={label=2020, value=2020}, summary=<p>Prof Hagan Bayley <span>|</span> University of Oxford<br>Dr Mark Kotter <span>|</span> Founder and CEO | bit.bio<br><br></p>, date_published=1706918400000, sort_date=1594857600000, tags=[{label=ioSkeletal Myocytes, value=ioSkeletal Myocytes}], media_contact=null, listing_button_label=Watch now}, {hs_name=Research in Motion with ioSkeletal Myocytes, hs_id=161968263518, hs_path=introducing-human-ipsc-derived-muscle-cells-for-research-and-drug-discovery, button_label=Watch webinar, button_link=https://www.youtube.com/watch?v=TBMR5YNucoMvingin, type={label=Webinar, value=Webinar}, thumbnail={alt_text=, width=738, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/BitBio2022/product-page/ioSkeletal-Myocytes-DDW-ICC-staining-header-image-comp.jpg, height=334}, year={label=2020, value=2020}, summary=<p>Dr Luke Flatt | Senior Scientist | Charles River Laboratories</p> <p>Dr Will Bernard | Senior Scientist | bit.bio</p> <p><span><br><br><br></span></p>, date_published=1707091200000, sort_date=1641340800000, tags=[{label=ioSkeletal Myocytes, value=ioSkeletal Myocytes}, {label=ioSkeletal Myocytes DMD44, value=ioSkeletal Myocytes DMD44}, {label=ioSkeletal Myocytes DMD52, value=ioSkeletal Myocytes DMD52}], media_contact=null, listing_button_label=Watch now}, {hs_name=Advancements in 3D modeling: Building mature, functional 3D skeletal muscle microtissues in vitro, hs_id=161968263527, hs_path=advancements-in-3d-modeling-building-mature-functional-3d-skeletal-muscle-microtissues-in-vitro, button_label=null, button_link=null, type={label=Webinar, value=Webinar}, thumbnail={alt_text=, width=1304, url=https://14527135.fs1.hubspotusercontent-na1.net/hubfs/14527135/Website%20content/Product%20pages/ioSkeletal%20Myocytes/bit.bio_ioskeletal%20myocytes%20About%20us.jpg, height=964}, year={label=2023, value=2023}, summary=<p>Dr Marieke Aarts | Principal Scientist | Bi/ond</p> <p>Amanda Turner | Senior Product Manager | bit.bio</p>, date_published=1710201600000, sort_date=1698710400000, tags=[{label=ioSkeletal Myocytes, value=ioSkeletal Myocytes}, {label=ioSkeletal Myocytes DMD44, value=ioSkeletal Myocytes DMD44}, {label=ioSkeletal Myocytes DMD52, value=ioSkeletal Myocytes DMD52}], media_contact=null, listing_button_label=Watch now}])

Poster

Human iPSC-derived DMD skeletal myocytes for 3D functional studies and dystrophin restoration

Bernard, et al

bit.bio

2024

Download

Brochure

ioSkeletal Myocytes

bit.bio

Download

User manual

ioSkeletal Myocytes and related disease models | User Manual

V5

bit.bio

2023

Download

Webinar

Advancements in 3D modeling: Building mature, functional 3D skeletal muscle microtissues in vitro

Dr Marieke Aarts | Principal Scientist | Bi/ond

Amanda Turner | Senior Product Manager | bit.bio

Watch now

Poster

Generation of 3D skeletal muscle microtissues using ioSkeletal Myocytes

Dr Mitchell Han

Bi/ond

2023

View

Publication

Functional neurological restoration of amputated peripheral nerve using biohybrid regenerative bioelectronics | Publication

Rochford and Carnicer-Lombarte et al.

Science Advances

2023

Featuring opti-ox enabled skeletal myocytes iPS cell line

Read more

Poster

Genotype and phenotype validation of an isogenic human iPSC-derived neuronal model of Huntington’s Disease

Oosterveen, et al

bit.bio

2022

View

Video

Introducing ioSkeletal Myocytes | Developing the next generation of human muscle cells

Dr Will Bernard | Director of Cell Type Development | bit.bio

Watch

Webinar

Research in Motion with ioSkeletal Myocytes

Dr Luke Flatt | Senior Scientist | Charles River Laboratories

Dr Will Bernard | Senior Scientist | bit.bio

Watch now

Talk

CRISPR and the Art of Perturbation Screening: Unbiased functional genomic screening meets the best human cellular models

Kam Dhaliwal | SVP Strategic Alliances | bit.bio

Talk at ELRIG CRISPR in Drug Discovery

Watch now

Talk

Consistent and scalable human iPSC-derived cells for in vitro disease modelling and drug discovery

Kam Dhaliwal |  SVP Strategic Alliances | bit.bio
Dr Thomas Moreau | Head of Research | bit.bio

Talk at ELRIG Drug Discovery Digital

Watch now

Publication

Reprogramming the stem cell for a new generation of cures

Davenport A, Frolov T & Kotter M

Drug Discovery World

2020

 

 

Read more

Webinar

Advances in cellular reprogramming: from stem cells to printed tissues

Prof Hagan Bayley | University of Oxford
Dr Mark Kotter | Founder and CEO | bit.bio

Watch now

References

1. Danisovic L, Culenova M, Csobonyeiova M (2018) Induced Pluripotent Stem Cells for Duchenne Muscular Dystrophy Modeling and Therapy. Cells. 2018 Dec 7;7(12):253. doi: 10.3390/cells7120253. PMCID: PMC6315586  PMID: 30544588

Related pages

Discover ioCells Learn about our range of human iPSC-derived cells for research and drug discovery

Resources Explore our latest scientific insights, webinars, blogs and videos

Our platform Discover the cell coding platform that powers our ioCells