protocol
RNA extraction and RT-qPCR protocol for ioCells
This protocol is recommended for characterising of ioCells at transcriptomic level, including RNA extraction and RT-qPCR instructions.
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This protocol is recommended for characterising of ioCells at transcriptomic level, including RNA extraction and RT-qPCR instructions.
RT-qPCR is a powerful and highly sensitive technique that uses fluorescent dyes or probes for the specific detection and quantification of RNA transcripts in a biological sample. As such, RT-qPCR can provide precise insights into the gene expression profiles and transcriptional activity of target genes.
For characterising iPSC-derived cells at the transcriptional level, scientists rely on tracking the expression of specific gene markers to confirm cell identity or assess cellular states. For example, the downregulation of pluripotency markers, such as POU5F1 (OCT4) and NANOG, alongside the robust upregulation of lineage-specific genes, provides a reliable molecular signature of cellular identity and maturity.