cat no | io1069, io1070, io1071
ioGlutamatergic Neurons PSEN1 M146L/M146L are opti‑ox deterministically programmed glutamatergic neurons carrying a genetically engineered homozygous M146L mutation in the PSEN1 gene encoding presenilin 1 protein. These cells offer a rapidly maturing, disease relevant system for investigating the role of PSEN1 M146L mutation in early-onset Alzheimer's disease (AD).
A selection of three clones is available, all genetically matched to the wild-type control, ioGlutamatergic Neurons. This disease model is part of an Alzheimer's disease panel of physiologically relevant human iPSC-derived cells that can be incorporated into translational research and drug discovery workflows. Additional mutations include heterozygous PSEN1 M146L, heterozygous and homozygous APP KM670/671NL and APP V717I.
Confidently investigate your phenotype of interest across multiple clones with our disease model clone panel. Detailed characterisation data (below) and bulk RNA sequencing data (upon request) help you select specific clones if required.
per vial
A maximum number of 20 vials applies. If you would like to order more than 20 vials, please contact us at orders@bit.bio.
Make True Comparisons
Pair the ioDisease Model Cells with the genetically matched wild-type ioGlutamatergic Neurons to investigate the impact of the PSEN1 missense mutation on early-onset AD.
Scalable
With opti-ox technology, we can make billions of consistently programmed cells, surpassing the demands of industrial workflows.
Quick
The disease model cells and isogenic control are experiment ready as early as 2 days post revival, and form structural neuronal networks at 11 days.
ioGlutamatergic Neurons PSEN1 M146L/M146L express neuron-specific markers comparably to the wild type control
ioGlutamatergic Neurons PSEN1 M146L/M146L form structural neuronal networks by day 11
ioGlutamatergic Neurons PSEN1 M146L/M146L demonstrate gene expression of neuronal-specific and glutamatergic-specific markers following deterministic programming
Disease-related PSEN1 is expressed in ioGlutamatergic Neurons PSEN1 M146L/M146L following deterministic programming
bit.bio
V11
bit.bio
2024
Professor Deepak Srivastava
Professor of Molecular Neuroscience and Group Leader, MRC Centre for Developmental Disorders
King’s College London
Emmanouil Metzakopian | Vice President, Research and Development | bit.bio
Javier Conde-Vancells | Director Product Management | bit.bio
Dr Ania Wilczynska | Head of Computational Genomics | Non-Clinical | bit.bio
Innovation showcase talk at ISSCR
Marius Wernig MD, PhD | Stanford
Mark Kotter, MD, PhD | bit.bio
Oosterveen, et al
bit.bio & Charles River Laboratories
2023
Mark Kotter | CEO and founder | bit.bio
Marius Wernig | Professor Departments of Pathology and Chemical and Systems Biology | Stanford University
Madeleine Garrett | Field Application Specialist | bit.bio
Ritsma, et al
Charles River Laboratories & bit.bio
2022
Raman, et al
bit.bio
2022
bit.bio | MaxWell Biosystems | Charles River Laboratories
2022
Pavinlek, et al
Frontiers in Psychiatry
2022
Using ioGlutamatergic Neurons
Bando, et al
Frontiers in Cellular and Infection Microbiology
2022
Using ioGlutamatergic Neurons
Mah, et al
Experimental Neurology
2022
Using ioGlutamatergic Neurons
Read this blog on glutamatergic neuron cell culture for our top tips on careful handling, cell plating and media changes to achieve success from the outset.
Further your disease research by pairing our wild type cells with isogenic disease models.