ioMicroglia are human induced pluripotent stem cell (iPSC)-derived microglia, deterministically programmed using opti-ox technology. Like all primary human microglia cells, ioMicroglia are adherent cells that form attachments to their surrounding environment. For many applications including flow cytometry, single cell RNAseq, and re-seeding for co-culture, microglia need to be detached from the plate. However, doing so is a delicate process and to ensure ioMicroglia remain viable, it is essential to perform gentle detachment from the culture dish.

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