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Protocol overview
Introduction
This protocol is recommended for lipid-based delivery of synthetic mRNA into ioGlutamatergic Neurons in 96-well plates. It is also applicable to all ioGlutamatergic Neurons disease model cell products.
Key benefits:
- High transfection efficiency at 24 h post-thaw using Lipofectamine™ Stem, with minimal cell stress
- Optimised for use in 96-well plates to facilitate scalable screening experiments
- Expression detectable within 24–48 h and sustained for up to 18 days, as demonstrated by transfection of Green Fluorescent Protein (GFP)
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