cat no | io1024
ioSensory Neurons are a highly pure, easy to use and functional in vitro nociceptor model, enabling reliable chronic nociceptive pain research and therapeutics development for peripheral neuropathies.
ioSensory Neurons are precision reprogrammed using opti-ox™ technology, meaning consistency is built-in. Within 7 days post-revival, ioSensory Neurons form a highly pure (>99%) sensory neuronal population with a defined nociceptor identity, characterised by the expression of the pan-sensory neuron markers, PRPH, BRN3A, ISL1 and TUBB3, and key markers of nociceptors, NTRK1 and TRPV1.
These cells display spontaneous activity and show a functional nociceptor phenotype, as demonstrated by responsiveness to selective agonists for TRPV1, TRPM3, and TRPM8.
Confidently investigate your phenotype of interest across multiple clones with our disease model clone panel. Detailed characterisation data (below) and bulk RNA sequencing data (upon request) help you select specific clones if required.
per vial
A maximum number of 20 vials applies. If you would like to order more than 20 vials, please contact us at orders@bit.bio.
Highly Pure
>99% pure sensory neurons with a defined nociceptor identity by day 7 post-revival, as confirmed by single cell RNA sequencing.
Functional
ioSensory Neurons display spontaneous activity and a functional nociceptor phenotype, as shown by calcium mobilisation in response to specific TRP agonists.
Easy to use
Cryopreserved cells arrive, programmed to mature rapidly upon revival. Simple two-step protocol using one medium – no mitomycin C treatment is necessary.
opti-ox precision reprogrammed ioSensory Neurons rapidly form a homogenous neuronal population
ioSensory Neurons express pan-sensory neuron-specific markers
ioSensory Neurons show visible neuronal networks by day 7
Single cell RNA-sequencing shows ioSensory Neurons form a pure population (>99%) of sensory neurons and express key pan-sensory neuronal markers
Single cell RNA-sequencing shows ioSensory Neurons express key nociceptor markers
ioSensory Neurons display enhanced TRPM3 and TRPM8 responses with bespoke media for these channels and increased culture length
Cells arrive ready to plate
Dr Ania Wilczynska | Head of Computational Genomics | Non-Clinical | bit.bio
Innovation showcase talk at ISSCR
Marius Wernig MD, PhD | Stanford
Mark Kotter, MD, PhD | bit.bio
Prof Roger Pedersen | Adjunct Professor and Senior Research Scientist at Stanford University
Dr Thomas Moreau | Director of Cell Biology Research | bit.bio
Download this infographic to find out how the approach used to generate human iPSC-derived cells influences purity, batch consistency and protocol speed.