Cell culture media for the culture of ioMicroglia up to 10 days post-thaw

cat no | io4130 Early Access

ioMicroglia Media Kit

Cell culture media for ioMicroglia

Cell culture media kit for the culture of ioMicroglia up to 10 days post-thaw
Compatible with all ioMicroglia wild-types and derivative products
Supports reliable cell culture following the ioMicroglia User Manual using validated reagents

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A maximum number of 20 kits applies. If you would like to order more than 20 kits, please contact us at orders@bit.bio.

Media kits for the culture of human iPSC-derived microglia

Learn more about ioMicroglia

The ioMicroglia Media Kit simplifies media preparation, enabling scientists to culture ioMicroglia for up to 10 days following the User Manual.

ioMicroglia are human iPSC-derived microglia deterministically programmed using opti-ox technology. By day 4 post-revival, they demonstrate key functional phenotypes, including cytokine release and phagocytosis.

The kit contains the majority of reagents required for the culture workflow. The few additional components needed are clearly outlined in the Media Kit Protocol.

Benchtop benefits

Easy-to-use

Provides the majority of components in a single kit, ensuring reliable quality and saving hands-on time in the lab.

Quick

Straightforward media preparation process that integrates seamlessly with the User Manual, enabling a quick and confident start to culturing cells.

Cost efficient

Eliminates the need to source individual reagents from multiple suppliers, protects your workflow from out-of-stock delays and reduces initial purchasing costs.

Product specifications

Kit components

Base Medium
Supplement A
Supplement B
Supplement C x2

Kit size

Small: for use with small vials, culture up to 10 days
Large: for use with large vials, culture up to 10 days

Quality control

Sterility, Performance of ioMicroglia culture to standard quality control specification

User storage

-20°C or -80°C for 6 months from date of manufacture

Shipping info

Dry ice

Product use

Media kits are for research use only

Get started with

Media Kit Protocol

Limited Use License & Statement of Use

How to culture ioMicroglia

In this video, our scientist will take you through the step-by-step process of how to thaw, seed and culture ioMicroglia.

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Product resources

Protocol

mRNA transfection of ioMicroglia

Lipid-based delivery of synthetic mRNA in 96-well plates

Phagocytosis assessment of ioMicroglia | bit.bio

Protocol

ioMicroglia: Assessing phagocytosis of pHrodo™ labelled bioparticles and Amyloid-β 1-42

Protocol

Stimulation for cytokine secretion in ioMicroglia

Protocol

Cell detachment protocol for ioMicroglia: re-seeding experiments

Protocol

Cell counting protocol for ioCells

Step-by-step to quantify the number of cells with 2 methods to determine cell viability

Protocol

Immunocytochemistry staining for ioMicroglia

Including fixation, permeabilisation, and antibody staining of key markers (with recommended antibodies)

Protocol

Cell detachment protocol for ioMicroglia: flow cytometry and single cell experiments

Protocol

Lentiviral transduction of ioMicroglia

Using lentiviral-based transduction

Protocol

ioMicroglia Media Kit protocol

For culturing ioMicroglia (and derivatives) up to 10 days post-thaw

Frequently Asked Questions (FAQs)

Does the ioMicroglia Media Kit contain everything needed to culture ioMicroglia?

The ioMicroglia Media Kit contains the majority of reagents required to culture the ioMicroglia portfolio of products. The following additional components must be purchased separately: ioMicroglia vials, poly-L-lysine (coating reagent), ß-mercaptoethanol, and doxycycline. Recommended suppliers and catalogue numbers can be found in the Media Kit Protocol.

Which ioMicroglia products are compatible with the media kits?

All ioMicroglia wild-types, disease models, CRISPR-ready and tracker cells can be used with the media kits, view the full list here.

How long can I store the ioMicroglia Media Kit before use?

The ioMicroglia Media Kit is stable for 6 months from the date of manufacture (DoM) when stored correctly at -20°C or -80°C and unopened. Check the DoM on receipt of the kit and ensure its use within the correct time period.

Can I use the ioMicroglia Media Kit for co-culture experiments?

The ioMicroglia Media Kit can only be used for monoculture of the ioMicroglia. For co- and tri-culture systems, refer to our validated protocols, which feature specialised media formulations tailored to support those specific cell combinations.

Which assays have been used to validate the functionality of ioMicroglia when cultured with the ioMicroglia Media Kit?

The functionality of ioMicroglia is evaluated using cytokine release upon stimulation and phagocytosis of bioparticles. Every batch of the ioMicroglia Media Kit undergoes validation using these functional assays to ensure consistent, reliable cell performance.

How many plates can be cultured with a single ioMicroglia Media Kit?

The plate coverage of the ioMicroglia Media Kit depends on the kit size and the chosen plate format:

Small media kit for use with small vials (>1.5 x 10⁶ cells): Supports the culture of one full 6-, 12-, 24-, 48-, or 96-well plate, OR half (0.5) of a 384-well plate* up to day 10 post-thaw.

Large media kit for use with large vials (>5 x 10⁶ cells): Supports the culture of three full 6- to 96-well plates, OR one and a half (1.5) 384-well plates* up to day 10 post-thaw.

*384-well plates require more media due to a higher surface area to volume ratio; in addition, remember to account for extra media requirements when using automated liquid handling systems.

What if I want to culture the ioMicroglia past day 10?

Ensure you purchase enough media kits to cover your planned experiments before starting. Always match the kit size (small or large) to your vial size. If you are culturing the ioMicroglia for a prolonged period of time, note that we also offer open source media formulations that can be found in the User Manual.

Can I combine media from this kit with media I made myself following the User Manual?

Do not use the media prepared using the ioMicroglia Media Kit in the same experiment as media prepared from individual components following the User Manual. To ensure experimental consistency and reproducibility, it is best to use a single media source throughout your entire workflow.

Can I use a large kit for a small vial of cells to increase the culture time?

To ensure optimal cell health, note that complete media has a 4-day shelf life at 2–8°C due to the limited stability of growth factors and small molecules. Because of this 4-day potency limit, excess complete media from a large kit cannot be stored for long-term use with smaller cell volumes. To support the full 10-day workflow without waste, always match the kit size (small or large) to your vial size.

Any other questions?

If your question is not covered here, our technical support team is available at technical@bit.bio.