cat no | io1021
ioMicroglia (io1021) are male donor-derived human microglial cells precision reprogrammed from iPSC, using opti-ox™ technology.
Within 10 days post-revival, ioMicroglia are ready for experimentation, expressing (>90%) key microglia markers, including TMEM119, P2RY12, IBA1, TREM2, CX3CR1, CD11b, CD45, and CD14.
ioMicroglia recapitulate key human microglia functions with lot-to-lot consistency, including mediating an inflammatory response, disposal of unwanted materials, and carrying out immune surveillance. In addition, ioMicroglia also display chemotaxis and can be co-cultured with ioGlutamatergic Neurons™ to gain insights into complex intercellular interactions.
ioMicroglia provide a functional, consistent, rapid, and easy-to-use hiPSC-based model for neurodegenerative disease research and drug development. These cells can be used in conjunction with our female iPSC donor-derived ioMicroglia (io1029) and related disease models for Alzheimer's to study the effect of donor and sex-related differences and disease relevant mutations on microglia phenotype, functionality, and drug response.
Confidently investigate your phenotype of interest across multiple clones with our disease model clone panel. Detailed characterisation data (below) and bulk RNA sequencing data (upon request) help you select specific clones if required.
per vial
A maximum number of 20 vials applies. If you would like to order more than 20 vials, please contact us at orders@bit.bio.
Functional
ioMicroglia display key phagocytic and cytokine secretion functions with lot-to-lot consistency.
Quick
Rapidly maturing male donor-derived human microglia that are ready to use within 10 days post-revival.
Co-culture compatible
Suitable for co-culture studies with neurons at 1 day post-thaw.
opti-ox precision reprogrammed ioMicroglia from a male donor rapidly form a homogenous microglia population.
Flow cytometry analysis of male donor-derived ioMicroglia shows key phenotypic marker expression
Male donor-derived ioMicroglia show ramified morphology by day 10
Key marker expression in male donor-derived ioMicroglia and ioGlutamatergic Neuron co-cultures
V7
bit.bio
2024
Prof Roger Pedersen | Adjunct Professor and Senior Research Scientist at Stanford University
Dr Thomas Moreau | Director of Cell Biology Research | bit.bio
Mark Kotter | CEO and founder | bit.bio
Marius Wernig | Professor Departments of Pathology and Chemical and Systems Biology | Stanford University
An interview with a leading researcher and microglia expert Dr Anthony Vernon at King's College Institute of Psychiatry, Psychology & Neuroscience, to demystify the complex roles of microglia in our brand new blog.