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ioGlutamatergic Neurons^™

Human iPSC-derived glutamatergic neurons

ioGlutamatergic Neurons have been precision reprogrammed from human induced pluripotent stem cells (iPSC) using opti-ox™ technology. Human stem cells, within days, convert into consistent, mature, functional glutamatergic neurons characterised to consistently show >80% expression of glutamate transporter genes VGLUT1 and VGLUT2.

Glutamatergic neurons are delivered cryopreserved and ready-to-culture making them a high-quality human model for fundamental research, disease modelling and drug discovery.

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Benchtop benefits

Quick

Ready for experimentation as early as 2 days post revival and form functional neuronal networks at 17 days.

Scalable

Industrial scale quantities at a price point that allows the cells to be used from research to screening scale.

Easy to use

Cells arrive programmed to rapidly mature upon revival. One medium is required in a two-step protocol.

Product information

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Starting material

Human iPSC line

Karyotype

Normal (46, XY)

Seeding compatibility

6, 12, 24, 48, 96 & 384 well plates

Shipping info

Dry ice

Donor

Caucasian adult male (skin fibroblast)

Vial size

Small: >1 x 10⁶ viable cells Large: >5 x 10⁶ viable cells

Quality control

Sterility, protein expression (ICC) and gene expression (RT-qPCR)

Differentiation method

opti-ox cellular reprogramming

Recommended seeding density

30,000 cells/cm²

User storage

LN2 or -150°C

Format

Cryopreserved cells

Product use

ioCells are for research use only

Applications

Drug discovery
Neurotoxicology
High throughput screening
CRISPR Screening
3D bioprinting

Ready within days

ioGlutamatergic Neurons generated by transcription factor-driven reprogramming of iPSCs using opti-ox technology

Time-lapse video capturing the rapid and homogeneous neuronal phenotype acquisition upon thawing of cryopreserved ioGlutamatergic Neurons. 7 day time course.

Highly characterised and defined

ioGlutamatergic Neurons express glutamatergic neuron-specific markers

MAP2

VGLUT2

DAPI

MERGE

Immunofluorescent staining on post-revival day 11 demonstrates homogenous expression of the pan-neuronal protein, MAP2 and glutamatergic neuron-specific transporter, VGLUT2.

ioGlutamatergic Neurons form structural neuronal networks by day 11

Day 1

Day 4

Day 7

Day 11

ioGlutamatergic Neurons mature rapidly and form structural neuronal networks over 11 days, when compared to the isogenic control. Day 1 to 11 post thawing; 100X magnification.

Rapid gain of functional activity

ioGlutamatergic Neurons display neuronal activity that matures over-time

Examples of MaxOne high-resolution multi electrode array (MEA) recordings of ioGlutamatergic Neurons in BrainPhys™ media. The activity maps show firing rate (A), spike amplitude (B) and % of active electrodes (C). Results demonstrate a time-dependent increase of spontaneous activity during neuronal maturation from 2 to 3 weeks post-revival.

Iovino, M. et al., 2019, Charles River Laboratories.

Cells demonstrate a time-dependent increase of spontaneous bursting activity over a three-week period

A
Percentage of active electrodes bursting

B
Example of a spontaneous spike

C
Example of a bursting phenotype

Click on the tabs to explore the data.

(A) The graph shows the % of active bursting electrodes for each time point. (B) An example of a spontaneous spike, taken at day 8 post-revival (1 second sweep, 32 µV/-18 µV). (C) An example of a bursting phenotype, taken at day 20 post-revival (1 second sweep, 16 µV/-16 µV). Cells were cultured in the bit.bio recommended open-source medium and recorded on 64-electrode MEAs.

NDimension (Science and Engineering) Ltd.

ioGlutamatergic Neurons co-cultured with rat-derived astrocytes demonstrate time-dependent increase in synchronous activity

Day 8

Day 13

Day 20

Click on the tabs to explore the data.

Array Wide Spike Detection Rate histograms (AWSDR – a graphical measure of synchrony) for 10-minute recordings on day 8, 13 and 20 post-revival ioGlutamatergic Neurons in co-culture with primary rat-derived astrocytes. Results show prominent synchronicity on day 13, exemplified by the ‘spikier’ nature of the associated AWSDR, which increases at day 20. Cells were cultured in the bit.bio recommended open-source medium and recorded on 64-electrode MEAs.

NDimension (Science and Engineering) Ltd.

Robust and scalable cells for high-throughput screening

ioGlutamatergic Neurons show good suitability for high-throughput screening in 384-well format plates

Cytotoxicity CellTiter-Glo®️ (CTG) and TR-FRET (HTRF®️) assays for AKT serine/threonine kinase 1 (AKT) and Huntingtin (HTT) proteins were performed on ioGlutamatergic Neurons in 384-well plates treated with tool compound (cmp) at day 9 post-revival. Compound titration results in a concentration response curve for all three assays (mean±sd of 2 replicates). CTG assay on ioGlutamatergic Neurons shows an excellent average signal/ background ratio and high suitability for HTS. HTRF® assays on ioGlutamatergic Neurons show lower signals but with low variability, and could therefore also provide a suitable platform for HTS.

Iovino, M. et al., 2019, Charles River Laboratories.

Industry leading seeding density

Do more with every vial

The seeding density of our human iPSC-derived glutamatergic neurons has been optimised and validated making it possible to have a cost point of under £0.67 (~$0.79) per well (96 well plate, seeding density 30,000 cells/cm², Large vial size).

This means scientists are able to do more with every vial and expand experimental design within budget without losing out on quality. Resulting in more experimental conditions, more repeats, and more confidence in the data.

One Small vial can plate a minimum of 0.7 x 24-well plate, 1 x 96-well plate, or 1.5 x 384-well plate.

One Large vial can plate a minimum of 3.6 x 24-well plate, 5.4 x 96-well plate, or 7.75 x 384-well plates.

Easy culturing

Cells arrive ready to plate

ioGlutamatergic Neurons are delivered in a cryopreserved format and are programmed to rapidly mature upon revival in the recommended media. The protocol for the generation of these cells is a three-phase process: 1. Induction (carried out at bit.bio) 2. Stabilisation for 4 days 3. Maintenance during which the neurons mature.

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Supporting documentation

FAQ

User Manual

Statement of Use

Product resources

Application note

Developing next-generation in vitro phenotypic assays for Huntington’s disease by combining a precision reprogrammed hiPSC-derived disease model with high-density microelectrode arrays

2022

bit.bio | MaxWell Biosystems | Charles River Laboratories

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Publication

Studying Neurodevelopmental Disorders with Stem Cells | Publication

Pavinlek., et al | Frontiers in Psychiatry

2022

Using ioGlutamatergic Neurons

Publication

Glutamatergic Neurons and Brain Cyst Formation | Publication

Bando., et al | Frontiers in Cellular and Infection Microbiology

2022

Using ioGlutamatergic Neurons

Publication

Stem Cells and Spinal Cord Injury Repair | Publication

Mah., et al | Experimental Neurology

2022

Using ioGlutamatergic Neurons

Webinar

Studying Neurodevelopment with Stem Cells | Webinar

Dr Deepak Srivastava | King’s College London

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Video

Drug Screening With Stem Cells and Charles River Part 2 | Video

Charles River

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Video tutorial

Culturing Glutamatergic Neurons | How-to Video

Dr Kaiser Karim | Scientist
bit.bio

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Video tutorial

Culture Vessel Prep For Glutamatergic Neurons | How-to Video

Dr Kaiser Karim | Scientist
bit.bio

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Giving you easy access to endless and reliable human cells

Read this blog to find out how our precision cellular reprogramming technology, opti-ox is powering cell identity, giving you easy access to endless and reliable human cells!

Read blog