User manual
CRISPR-Ready ioMicroglia
Human iPSC-derived microglia expressing Cas9 and dCas9 for CRISPR knockout, activation or interference.
Human iPSC-derived microglia expressing Cas9 and dCas9 for CRISPR knockout, activation or interference.
CRISPR-Ready ioMicroglia rapidly mature into functional microglia after revival in the recommended medium. These cells constitutively express Cas9 or dCas9 nucleases, allowing users to perform single-gene knockouts, gene activation or gene intereference, pooled or arrayed CRISPR screens.
This user manual contains detailed instructions to culture the following products:
- CRISPRko-Ready ioMicroglia | io1094
- CRISPRa-Ready ioMicroglia | io1102
- CRISPRi-Ready ioMicroglia | io1103
The document provides full details for cell culture and guide RNA delivery by either lentivirus transduction or lipid-based transfection and includes:
- Culture of CRISPR-Ready ioMicroglia portfolio
- Lipid-based delivery of synthetic sgRNA
- Lentiviral delivery of sgRNA
- Flow cytometry readout
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