User manual
CRISPRa-Ready ioMicroglia User Manual
cat no io1102 Early Access | Human iPSC-derived microglia expressing catalytically inactive Cas9 nuclease fused to a transcriptional activation domain for gene activations.
cat no io1102 Early Access | Human iPSC-derived microglia expressing catalytically inactive Cas9 nuclease fused to a transcriptional activation domain for gene activations.
CRISPR activation (CRISPRa)-Ready ioMicroglia constitutively express catalytically inactive Cas9 nuclease (dCas9) fused to a transcriptional activation domain for gene activations, pooled or arrayed CRISPR activation screens.
The ioMicroglia portfolio are delivered in a cryopreserved format and are programmed to rapidly mature upon revival in the recommended media.
Guide RNA delivery
The user manual includes protocols for guide RNA delivery and flow cytometry readout.
Complete the form to download the user manual.
The ioMicroglia portfolio are delivered in a cryopreserved format and are programmed to rapidly mature upon revival in the recommended media.
Guide RNA delivery
The user manual includes protocols for guide RNA delivery and flow cytometry readout.
- Phase 1 - Stabilisation (day 0 to 1)
The ioMicroglia are revived at the user’s laboratory using the recommended medium supplemented with doxycycline & ROCK inhibitor (24 h) for sustained induction.
- Phase 2 - Maturation (day 1 to 10)
The ioMicroglia require full media change switching to a maturation medium with the removal of doxycycline & ROCK inhibitor.
- Phase 3 - Maintenance (day 10 onwards)
Depending on assay requirements, the ioMicroglia can be used over different lengths of time in the maintenance medium.
Complete the form to download the user manual.
DOC-4238 1.0