A human iPSC-derived neuron and glioma culture model reveals increased neuronal excitability and loss of GABAergic inhibition
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The establishment of a human iPSC-derived culture comprising glutamatergic neurons, GABAergic neurons and astrocytes, with and without glioma cells
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How the neuronal cultures developed mature synaptic architecture, spontaneous coordinated network activity and functional GABAergic signalling, characterised by multi-electrode array (MEA) recordings and whole-cell patch-clamp electrophysiology
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How glioma incorporation increased spike rate and coordinated network activity, and reversed the response to the GABA-A agonist muscimol from suppression to elevation of neuronal firing
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How whole-cell patch-clamp recordings demonstrated increased intrinsic excitability of glutamatergic neurons following glioma co-culture
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How ioGlutamatergic Neurons, GFP ioGlutamatergic Neurons and ioGABAergic Neurons were used
Haile H, et al.
Research Square | Preprint
2026