A human neuron-microglia tri-culture platform to study the influence of microglia on developing neuronal networks in vitro

Read the publication to explore:

• The establishment of a rapid and reproducible human tri-culture platform including glutamatergic neurons, GABAergic neurons, and microglia

• Automated high-content imaging confirming the formation of both excitatory and inhibitory synapses

• Multi-electrode (MEA) records identifying an 80:20 glutamatergic-to-GABAergic ratio as the most robust configuration for sustained and reproducible network activity

• The incorporation of microglia carrying the Alzheimer’s disease-associated TREM2 R47H mutation as a proof of concept, revealing reproducible alterations in neuronal burst dynamics

• How ioGlutamatergic Neurons, ioGABAergic Neurons, ioMicroglia and ioMicroglia TREM2 R47H/R47H were used