UPCOMING! Immunocytochemistry workshop | Top tips for human iPSC-derived cells

Immunocytochemistry (ICC) is a powerful technique to study protein expression, localisation and cell morphology in human iPSC-derived cells. It is widely used to confirm the presence of key marker proteins, validate targets in drug discovery, and perform phenotypic screening, such as quantifying changes in synaptic proteins.

Get practical advice and considerations for successfully applying ICC to iPSC-derived cells.

Key learning points

• The importance of coating reagents, culture surfaces and seeding density 
• Critical protocol steps such as fixation and permeabilisation, highlighting optimised approaches developed at bit.bio
• Selecting the most suitable antibody and optimising antibody concentrations to balance sensitivity and reproducibility in your experiments
• Options for image acquisition across different platforms, including fluorescence, high-content imaging, and confocal microscopy
• Guidance on image analysis
• Common troubleshooting tips 

Date | 14th October 2025

Time | 8am PDT | 11am EDT | 4pm BST | 5pm CEST