User manual
CRISPRko-Ready ioOligodendrocyte-like cells
cat no io1095 | Human iPSC-derived oligodendrocyte-like cells expressing Cas9 for rapid gene knockout generation
cat no io1095 | Human iPSC-derived oligodendrocyte-like cells expressing Cas9 for rapid gene knockout generation
CRISPRko-Ready ioOligodendrocyte-like cells are delivered in a cryopreserved format. The user manual for the generation of these cells follows a simple two-phase process:
Induction
Carried out at bit.bio before distribution.
Phase 1 - Stabilisation (24 hours)
Cells are revived at the user’s laboratory using the recommended medium.
Phase 2 - Maturation (from day 1 to day 8)
Cells mature into oligodendrocyte-like cells by using the recommended medium. gRNA delivery was tested on day 2 post-revival.
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V1
2025
bit.bio