User manual

CRISPRko-Ready ioOligodendrocyte-like cells

cat no io1095 | Human iPSC-derived oligodendrocyte-like cells expressing Cas9 for rapid gene knockout generation
CRISPRko-Ready ioOligodendrocyte-like cells
cat no io1095 | Human iPSC-derived oligodendrocyte-like cells expressing Cas9 for rapid gene knockout generation

CRISPRko-Ready ioOligodendrocyte-like cells are delivered in a cryopreserved format. The user manual for the generation of these cells follows a simple two-phase process:

Induction

Carried out at bit.bio before distribution.

Phase 1 - Stabilisation (24 hours)

Cells are revived at the user’s laboratory using the recommended medium.

Phase 2 - Maturation (from day 1 to day 8)

Cells mature into oligodendrocyte-like cells by using the recommended medium. gRNA delivery was tested on day 2 post-revival.

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V1
2025
bit.bio

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