Rapid and consistent generation of functional microglia from reprogrammed hiPSCs to study neurodegeneration and neuroinflammation

In this poster, you will explore:

• How consistent, functional ioMicroglia can be generated from cryopreserved vials in just 10 days
• Flow cytometry, and immunocytochemistry analysis of key microglia marker expression, including TMEM119, P2RY12, CD14, CD45 and CD11b
• Phagocytosis assays showing that ioMicroglia undergo phagocytosis of E. coli and amyloid beta particles
• Cytokine secretion assays showing that ioMicroglia secrete pro-inflammatory cytokines when exposed to LPS and IFNγ
• Immunocytochemistry data showing ioMicroglia in co-culture with ioGlutamatergic Neurons
• Single cell and bulk RNA-sequencing data showing that ioMicroglia are enriched for key phenotypic markers, and expression profiles closely cluster with primary adult and foetal microglia