CRISPR screens in iPSC-derived human neurons and glia for target identification and validation

You will learn:

• CRISPR-Ready opti-ox iPSC models enable precise, scalable functional genomics in human CNS cell types by providing homogeneous populations that stably express Cas9 effector modules

• A diverse range of CNS cell types, including ioGlutamatergic Neurons, ioMicroglia, and ioOligodendrocyte-like cells, can be used for cross-cell-type dissection of gene function using a universal, disease-relevant gRNA library

• An end-to-end workflow for target discovery integrates pooled CRISPRa/i/ko screens with single-cell RNA-seq and functional assays in human neural systems