ioMotor Neurons are delivered in a cryopreserved format and are programmed to rapidly mature upon revival in the recommended media. The user manual for the generation of these cells is a simple three-phase process:
- Phase 1 – Stabilisation (day 0 to 2)
The ioMotor Neurons are revived at the user’s laboratory using the recommended medium supplemented with doxycycline for sustained induction.
- Phase 2 – Pre-Maintenance (day 2 to 4)
The ioMotor Neurons require a full media change with the recommended medium with doxycycline.
- Phase 3 – Maintenance (day 4 onwards)
The ioMotor Neurons require full media change with the recommended medium without doxycycline. Half media changes are subsequently performed every 48 hours onwards
Depending on assay requirements, ioMotor Neurons can be used over different lengths of time in the maintenance medium.
Complete the form to download the ioMotor Neuron user manual.