technical note
Enhancement of MBP expression in human iPSC-derived oligodendrocyte-like cells by optimising db-cAMP concentration in media
In this application note, ioOligodendrocyte-like cells (ioOLCs) are characterised following a systematic evaluation of media components, specifically the addition of dibutyryl cyclic adenosine monophosphate (db-cAMP). The addition of db-cAMP is shown to enhance Myelin Basic Protein (MBP) expression in a concentration-dependent manner while promoting maturation and long-term culture stability of the cell culture. This contributes to the relevance of ioOLCs in demyelinating disease studies, offering a simple, quick human oligodendroglial model.
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In this application note, ioOligodendrocyte-like cells (ioOLCs) are characterised following a systematic evaluation of media components, specifically the addition of dibutyryl cyclic adenosine monophosphate (db-cAMP). The addition of db-cAMP is shown to enhance Myelin Basic Protein (MBP) expression in a concentration-dependent manner while promoting maturation and long-term culture stability of the cell culture. This contributes to the relevance of ioOLCs in demyelinating disease studies, offering a simple, quick human oligodendroglial model.