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bit.bio_GFP_ioMotorNeurons_HERO_GFP_D11
bit.bio_GFP_ioMotorNeurons_FACS_Analysis_GFP_WT_vs_GFP_D1_D11_D21
bit.bio_GFP_ioMotorNeurons_IF_Panel_D11_DAPI_GFP_CHAT_ISL2-1
bit.bio_GFP_ioMotorNeurons_IF_Panel_D11_DAPI_MAP2_VACHT_HB9-1
bit.bio_GFP_ioMotorNeurons_vs_WT_Brightfield_D1_D4_D11_D14
bit.bio_GFP_ioMotorNeurons_vs_WT_RTqPCR_OCT4_TUBB3_MAP2_CHAT_VACHT_HB9_ISL2
bit.bio_GFP_ioMotorNeurons_HERO_GFP_D11
bit.bio_GFP_ioMotorNeurons_FACS_Analysis_GFP_WT_vs_GFP_D1_D11_D21
bit.bio_GFP_ioMotorNeurons_IF_Panel_D11_DAPI_GFP_CHAT_ISL2-1
bit.bio_GFP_ioMotorNeurons_IF_Panel_D11_DAPI_MAP2_VACHT_HB9-1
bit.bio_GFP_ioMotorNeurons_vs_WT_Brightfield_D1_D4_D11_D14
bit.bio_GFP_ioMotorNeurons_vs_WT_RTqPCR_OCT4_TUBB3_MAP2_CHAT_VACHT_HB9_ISL2

cat no | io1112 Early Access

GFP ioMotor Neurons

Human iPSC-derived motor neurons constitutively expressing GFP

  • Cryopreserved human iPSC-derived cells powered by opti-ox that are ready for experiments in days
  • Motor Neurons constitutively expressing GFP, easy to track in multi-cell cultures, ideal for live-cell imaging
  • Co-culture compatible with ioSkeletal Myocytes and astrocytes

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bit.bio_GFP_ioMotorNeurons_HERO_GFP_D11

Human iPSC-derived motor neurons constitutively expressing GFP

bit.bio_GFP_ioMotorNeurons_FACS_Analysis_GFP_WT_vs_GFP_D1_D11_D21

Flow cytometry analysis of GFP expression at day 1, day 11 and day 21

Flow cytometry analysis demonstrating GFP expression in >99% of cells for GFP ioMotor Neurons at Day 1 (centre-left) and Day 11 (centre-right). No GFP expression seen in wild-type ioMotor Neurons (left). At day 21, the percentage of cells expressing GFP has not decreased, indicating there is no silencing of the reporter gene (right). 

bit.bio_GFP_ioMotorNeurons_IF_Panel_D11_DAPI_GFP_CHAT_ISL2-1

GFP ioMotor Neurons exhibit comparable expression of neuron-specific markers to the wild-type control

Immunofluorescent staining 11 days post-revival shows similar homogenous expression of cholinergic marker (ChAT), and motor neuron marker (ISL2) in both GFP ioMotor Neurons and the wild-type control.

The GFP signal is visible exclusively in GFP ioMotor Neurons and absent in the wild-type control.

bit.bio_GFP_ioMotorNeurons_IF_Panel_D11_DAPI_MAP2_VACHT_HB9-1

GFP ioMotor Neurons exhibit comparable expression of neuron-specific markers to the wild-type control

Immunofluorescent staining 11 days post-revival shows similar homogenous expression of pan-neuronal marker (MAP2), cholinergic marker (VAChT), and motor neuron marker (HB9) in both GFP ioMotor Neurons and the wild-type control.

bit.bio_GFP_ioMotorNeurons_vs_WT_Brightfield_D1_D4_D11_D14

GFP ioMotor Neurons form a homogenous neuronal network by day 4, without clumping

GFP ioMotor Neurons mature rapidly, show motor neuron morphology and form structural neuronal networks over 14 days with no sign of clumping of coalescing of cell bodies. Day 1 to 14 post thawing. 400 µm scale bar. 

bit.bio_GFP_ioMotorNeurons_vs_WT_RTqPCR_OCT4_TUBB3_MAP2_CHAT_VACHT_HB9_ISL2

GFP ioMotor Neurons demonstrate gene expression of neuronal-specific and motor-neuron-specific markers following deterministic programming

Gene expression analysis demonstrates that GFP ioMotor Neurons (GFP) and wild-type ioMotor Neurons (WT) lack the expression of pluripotency marker OCT4 (POU5F1) at day 11, while robustly expressing pan-neuronal (TUBB3 and MAP2), motor-neuron-specific (HB9 and ISL2) markers, as well as cholinergic markers (CHAT & VACHT).

Gene expression levels were assessed by RT-qPCR, data normalised to HMBS; cDNA samples of the parental human iPSC line (iPSC) were included as reference. Data represents day 11 post-revival samples.

Vial limit exceeded

A maximum number of 20 vials applies. If you would like to order more than 20 vials, please contact us at orders@bit.bio.

Human iPSC-derived motor neurons constitutively expressing GFP

GFP ioMotorNeurons are a fluorescent human lower motor neuronal model derived from our well-established wild-type ioMotor Neurons. These cells are engineered to constitutively express green fluorescent protein (GFP), and provide a powerful, ready-to-use tool for diverse applications.

Stable GFP expression enables easy, real-time tracking in complex, multi-cellular systems, facilitating the study of cellular interactions with glia, or neuro-muscular interactions when cultured with our ioSkeletal Myocytes. The cells are ideal for live cell imaging for assessment of neurite  outgrowth, neuronal morphology and survival in response to compound treatment.

GFP ioMotor Neurons are delivered cryopreserved and ready-to-culture. This eliminates the time and effort required to engineer your own GFP-expressing iPSC lines and manage complex directed differentiation protocols.

Benchtop benefits

quick_13

Live-cell imaging ready

Assess neurite outgrowth, neuronal morphology and survival in real-time.

culture_9

Co-culture compatible

Easily track GFP motor neurons in complex cultures with glia or skeletal myocytes.

easy_13

Easy-to-use

Within 4 days post revival cells are ready for experimentation, displaying motor neuronal morphology, without clumping.

Cells arrive ready to plate


bit.bio_ioMotorNeurons_user_manual_timelines_D11+

GFP ioMotor Neurons are delivered in a cryopreserved format and are programmed to rapidly mature upon revival in the recommended media.

Product specifications

Download product brochure

Starting material

Human iPSC line

Seeding compatibility

6, 12, 24, 96 & 384 well plates

Shipping info

Dry ice

Donor

Caucasian adult male, age 55-60 years old (skin fibroblast),
Genotype APOE 3/4

Vial size

Small: >1 x 10⁶ viable cells

Quality control

Sterility, protein expression (ICC), gene expression (RT-qPCR) and GFP expression (flow cytometry)

Differentiation method

opti-ox deterministic cell programming

Recommended minimum seeding density

30,000 cells/cm²

User storage

LN2 or -150°C

Format

Cryopreserved cells

Product use

ioCells are for research use only

Applications

Live-cell imaging
Co-culture
High-content screening
Neurotoxicology
Drug discovery

 

Scale your study with volume pricing

Enabling scientists to use human cells in their research, running additional experiments without rationing cells or limiting experimental scale

 

Order quantity Total vials received Pricing tier 
1 - 9 packs 3 - 27 vials Standard price
10 - 33 packs 30 - 99 vials Automatic 10% discount
> 34 packs > 100 vials > Contact us for a quote

 

Academic pricing: Academic users can purchase any ioCells in 3-vial packs ($/€/£ 999 per pack), available year-round with any cell type combination.

ioMotor Neurons Customer Testimonials

An image of Dr Irit Reichenstein

Dr Irit Reichenstein

Senior Scientist | Anima Biotech

"We did use the cells and were very happy with them :) They were very homogenous (unlike motor neurons from other vendors that we worked with), viable and absolutely beautiful. We got wonderful results with them."

An image of Dr Elizabeth Di Lullo

Dr Elizabeth Di Lullo

Associate Scientific Director | Brainever

“We thawed the cells and they looked wonderful. Close to 100% viable and looked great in cell culture! No clumping and easy to culture.”
Technical data

Highly characterised and defined

Flow cytometry analysis of GFP expression at day 1, day 11 and day 21

bit.bio_GFP_ioMotorNeurons_FACS_Analysis_GFP_WT_vs_GFP_D1_D11_D21

Flow cytometry analysis demonstrating GFP expression in >99% of cells for GFP ioMotor Neurons at Day 1 (centre-left) and Day 11 (centre-right). No GFP expression seen in wild-type ioMotor Neurons (left). At day 21, the percentage of cells expressing GFP has not decreased, indicating there is no silencing of the reporter gene (right).

GFP ioMotor Neurons exhibit comparable expression of neuron-specific markers to the wild-type control

bit.bio_GFP_ioMotorNeurons_IF_Panel_D11_DAPI_GFP_CHAT_ISL2-1
bit.bio_GFP_ioMotorNeurons_IF_Panel_D11_DAPI_MAP2_VACHT_HB9-1

Immunofluorescent staining 11 days post-revival shows similar homogenous expression of pan-neuronal markers (MAP2 & TUBB3), cholinergic markers (ChAT & VAChT), and motor neuron markers (HB9 & ISL2) in both GFP ioMotor Neurons and the wild-type control.

The GFP signal is visible exclusively in GFP ioMotor Neurons and absent in the wild-type control.

GFP ioMotor Neurons form a homogenous neuronal network by day 4, without clumping

bit.bio_GFP_ioMotorNeurons_vs_WT_Brightfield_D1_D4_D11_D14

GFP ioMotor Neurons mature rapidly, show motor neuron morphology and form structural neuronal networks over 14 days with no sign of clumping of coalescing of cell bodies. Day 1 to 14 post thawing. 400 µm scale bar.

GFP ioMotor Neurons demonstrate gene expression of neuronal-specific and motor-neuron-specific markers following deterministic programming

bit.bio_GFP_ioMotorNeurons_vs_WT_RTqPCR_OCT4_TUBB3_MAP2_CHAT_VACHT_HB9_ISL2

Gene expression analysis demonstrates that GFP ioMotor Neurons (GFP) and wild-type ioMotor Neurons (WT) lack the expression of pluripotency marker OCT4 (POU5F1) at day 11, while robustly expressing pan-neuronal (TUBB3 and MAP2), motor-neuron-specific (HB9 and ISL2) markers, as well as cholinergic markers (CHAT & VACHT).

Gene expression levels were assessed by RT-qPCR, data normalised to HMBS; cDNA samples of the parental human iPSC line (iPSC) were included as reference. Data represents day 11 post-revival samples.

How to culture ioMotor Neurons

In this video, our scientist will take you through the step-by-step process of how to thaw, seed and culture ioMotor Neurons.

Product resources

ioMotor Neurons and disease models user manual | bit.bio User manual
ioMotor Neurons and disease models user manual | bit.bio

DOC-2862 v2.0

bit.bio

2025

Download
CRISPRko-Ready ioMotor Neurons user manual | bit.bio User manual
CRISPRko-Ready ioMotor Neurons user manual | bit.bio

DOC-3073 V2
2025
bit.bio

Download
Mastering Cell Identity In A Dish: The Power Of Cellular Reprogramming | bit.bio Webinar
Mastering Cell Identity In A Dish: The Power Of Cellular Reprogramming | bit.bio

Prof Roger Pedersen | Adjunct Professor and Senior Research Scientist at Stanford University 

Dr Thomas Moreau | Director of Cell Biology Research | bit.bio

Watch now
Empowering motor neuron disease research and drug discovery with a new class of functional, reproducible hiPSC-derived motor neurons | bit.bio Webinar
Empowering motor neuron disease research and drug discovery with a new class of functional, reproducible hiPSC-derived motor neurons | bit.bio

Tom Brown | Senior Product Manager | bit.bio

Marcos Herrera Vaquero, PhD | Senior Scientist | bit.bio
Watch now
Harnessing AI-guided visual biology to discover drug targets for neurodegenerative disease | bit.bio Webinar
Harnessing AI-guided visual biology to discover drug targets for neurodegenerative disease | bit.bio

Ben Bar-Sadeh, PhD | Senior Scientist | Anima Biotech

Tom Brown | Senior Product Manager | bit.bio

Watch now

Expand your research

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Access 14 ALS and FTD disease models with disease-related mutations such as SOD1, FUS, MAPT and TDP-43 (TARDBP) genetically engineered in ioGlutamatergic Neurons and ioMotor Neurons.

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Interested in gene knockouts and CRISPR screens?
CRISPRko-Ready ioMotor Neurons cells are engineered to constitutively express Cas9 nuclease for the quick and easy generation of gene knockouts and CRISPR screens.

Explore CRISPRko-Ready ioMotor Neurons.

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Study neuronal networks and the impact of ALS-disease-related mutations by co-culturing ioMotor Neurons with astrocytes. Access 14 disease models and the single co-culture protocol for MEA.

View the co-culture protocol

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Explore ioAstrocytes

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Generate custom disease models or reporter lines
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Build your custom disease model or reporter line to pair with wild-type ioMotor Neurons as the genetically matched control.

Throughout the custom process, our experts will bring your project to life, and be on hand to support you with any technical queries.

Start the conversation today 

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Human iPSC-derived cells

powered by opti-ox

Consistent. Defined. Scalable.

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