Video tutorial
Morphology guide | ioSkeletal Myocytes
Know what to expect at every stage. This guide documents the morphological changes of ioSkeletal Myocytes from post-thaw recovery to a dense, aligned culture of elongated, spindle-shaped myocytes.
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Know what to expect at every stage. This guide documents the morphological changes of ioSkeletal Myocytes from post-thaw recovery to a dense, aligned culture of elongated, spindle-shaped myocytes.
The guide contains phase-contrast images from four key time points, with a low-magnification overview and a zoomed-in view at each one:
- Day 1 | Recovery phase: Triangular or slightly elongated cells, mostly clustered, with some rounded cells.
- Day 3 | Early network: Spindle-like cells begin aligning in parallel.
- Day 5 | Established culture: Uniform, aligned arrangement with less visible nuclei.
- Day 10 | Assay ready: Dense, aligned cells cover the plate, with occasional spontaneous contraction.
The guide also includes a healthy vs unhealthy culture comparison and expert handling tips.
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