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Morphology guide | ioMicroglia
Know what to expect at every stage. This guide documents the morphological changes of ioMicroglia from post-thaw recovery to an evenly distributed culture of ramified cells.
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Know what to expect at every stage. This guide documents the morphological changes of ioMicroglia from post-thaw recovery to an evenly distributed culture of ramified cells.
The guide contains phase-contrast images from four key time points, with a low-magnification overview and a zoomed-in view at each one:
- Day 1 | Recovery phase: Heterogeneous amoeboid, elongated and rounded cells with heterogeneous cytoplasmatic extensions.
- Day 4 | Assay ready: Increased cytoplasmic spreading, with ramified forms.
- Day 7 | Established culture: More ramified cells with distinct lateral micro-processes.
- Day 10 | Mature: Evenly distributed, predominantly ramified cells with distinct somas.
The guide also includes a healthy vs unhealthy culture comparison and expert handling tips.
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