A calcium imaging pipeline to detect and quantify compound-specific effects in human and mouse astrocytes and astrocyte-neuron cocultures

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• A simple automated calcium imaging pipeline to characterise changes in astrocytic calcium signalling

• A comparison of calcium responses in mouse astrocyte monocultures and astrocyte-neuron cocultures with human astrocytes differentiated from two different iPSC lines

• How lysergic acid diethylamide (LSD) decreased activity in mouse cocultured astrocytes, but increased activity in human astrocytes

• How human recombinant Tau oligomers, an in vitro model of Alzheimer’s disease pathology, decreased activity in both mouse and human astrocytes

• How ioAstrocytes were used