EdU proliferation protocol for ioOPC-like cells

Oligodendrocyte progenitor cells (OPCs) have the capacity to proliferate as well as to differentiate into mature oligodendrocytes. As proliferation is a key characteristic of OPCs, we have adapted the EdU labelling protocol to determine the proliferation rate of our ioOPC-like cells, which can be expanded and passaged for up to two weeks.

This protocol is based on the manual for the Click-iT™ EdU Cell Proliferation Kit for Imaging, Alexa Fluor™ 488 dye (ThermoFisher, cat no C10337), which was adapted by bit.bio for the quantification of EdU-positive ioOPC-like cells.