Optimisation of mRNA delivery to overcome transfection challenges in hiPSC-derived neurons and microglia

An mRNA-based approach enables efficient transfection of human iPSC-derived neurons and microglia, achieving ~60-95% efficiency with minimal toxicity and long-term sustained expression. High efficiency with high viability transfection requires systematic optimisation of seeding density, timepoint of transfection and mRNA/reagent amount.

bit.bio provides optimised, robust and reproducible mRNA transfection protocols for ioMotor Neurons, ioGlutamatergic Neurons, ioSensory Neurons and ioMicroglia, delivering high efficiency with minimal toxicity.