Rapid and consistent generation of human iPSC-derived oligodendrocyte-like cells using opti-ox technology

We have developed hiPSC-derived oligodendrocyte-like cells, which resemble a pre-myelinating oligodendrocyte state. The cells can be used as a valuable tool to study neurodegenerative and demyelinating diseases, where oligodendroglial cells are known to play a critical role.

Upon deterministic programming, cells rapidly acquire an OPC-like morphology and mature towards an oligodendrocyte-like morphology with multiple branched processes. ioOligodendrocyte-like cells express key oligodendroglia markers and show signs of maturation by day 8, as seen by ICC, qRT-PCR, and/or scRNAseq. Moreover, by day 8, scRNAseq analysis shows that 35% of the cells are MBP positive.