Generation and functional characterisation of motor neurons derived through transcription factor mediated programming of human pluripotent stem cells

Human induced pluripotent stem cells (hiPSCs) can be used to generate motor neurons for in vitro applications, however current differentiation protocols are often lengthy, inconsistent, and difficult to scale. We have used opti-ox technology to rapidly reprogram hiPSCs into motor neurons, termed ioMotor Neurons, which are a homogenous population of cells with classical neuronal morphology and neurite outgrowth.

• ioMotor Neurons can be generated with high consistency, as shown by bulk RNA-sequencing, and express key motor neuron already from day 4 post-revival.
• ioMotor Neurons offer rapid functionality – spontaneous activity is demonstrated by HD-MEA. Mono-cultures are devoid of activity due to high purity resulting in a lack of synapse formation.
• Electrical stimulation of ioMotor Neurons shows response to stimulus. Further, as voltage increases, as does the activity and intensity of the culture.