Poster

Stimulating 3D Skeletal Muscle Microtissues in a Novel Perfusable Microphysiological System with Integrated Electrodes

This poster, originally presented by Bi/ond at the Microphysiological Systems World Summit, Berlin, June 2023, shows how bit.bio’s skeletal muscle cells can be cultured successfully on the Bi/ond microchips to generate 3D skeletal muscle microtissues.
View poster
Stimulating 3D Skeletal Muscle Microtissues in a Novel Perfusable Microphysiological System with Integrated Electrodes
This poster, originally presented by Bi/ond at the Microphysiological Systems World Summit, Berlin, June 2023, shows how bit.bio’s skeletal muscle cells can be cultured successfully on the Bi/ond microchips to generate 3D skeletal muscle microtissues.

Bi/ond have developed the MUSbit, a novel microphysiological system with a microfluidic channel for perfusion, and pillars designed for anchoring muscle cell bundles. bit.bio’s human iPSC-derived skeletal muscle cells were encapsulated within a 3D extracellular matrix hydrogel on the MUSbit chip and cultured over 14 days, resulting in 3D microtissues that expressed muscle cell markers and contracted in response to electrical stimulation.

Download the poster to explore:

  • Successful 3D muscle bundle formation on the MUSbit microchip
  • Mature cross-striated muscle bundles expressing muscle cell markers
  • Robust twitch and tetanus contraction following electrical stimulation
  • Features of the MUSbit microchip and the prototype Let-it-bit microchip with functional integrated electrodes

The data demonstrates the successful generation of engineered 3D skeletal muscle microtissues using bit.bio’s ioSkeletal Mycoytes on the Bi/ond microchips, which have the capability for perfusion of muscle tissues and integration of functional electrodes. The bit.bio cells and Bi/ond microphysiological systems combine to offer a system for measuring muscle tissue contractile performance and investigating pharmacological interventions in wild-type and disease model cells, such as bit.bio’s human iPSC-derived Duchenne muscular dystrophy models with a DMD exon 44 deletion or DMD exon 52 deletion.

View poster

View poster

Related pages

News Read our latest updates and press coverage
Our platform Discover the cell coding platform behind our cells
Join us Explore the latest roles at bit.bio