Talk
Using human iPSC-derived ioSkeletal Myocytes and ioMotor Neurons to model complex neuromuscular systems in vitro
Dr Grace Cooper, bit.bio, showcased a 28-day neuromuscular co-culture using ioMotor Neurons and ioSkeletal Myocytes, demonstrating its use for studying NMJ formation and disease processes in both standard and microfluidic formats.
Dr Grace Cooper, bit.bio, showcased a 28-day neuromuscular co-culture using ioMotor Neurons and ioSkeletal Myocytes, demonstrating its use for studying NMJ formation and disease processes in both standard and microfluidic formats.
Given the success of ioSkeletal Myocytes (ioSKM) and ioMotor Neurons (ioMTN), bit.bio has begun development of more advanced neuromuscular cultures. In this User Exchange we will showcase an established 28 day co-culture of ioSKM and ioMTN in a widely accessible culture format. We will explore both the successes and technical considerations of developing a neuromuscular co-culture, and provide insights into this fully ioCell-derived in vitro model. This culture methodology lays the groundwork for exploring neuromuscular junction (NMJ) formation and cellular processes such as neurodegeneration, synaptic development and axon guidance. Therefore, in collaboration with NETRI we have initiated ioMTN/ioSKM co-culture in the DuaLink microfluidic system. This work aims to build more physiologically relevant ioMTN and ioSKM interactions in a microelectrode array compatible system.