3D neuroinflammation models using human iPSC-derived CNS cells to advance drug discovery
This webinar showcases human iPSC-derived CNS models used to study neuroinflammation across 2D and 3D systems.
The session opens with 2D microglia models developed at Medicines Discovery Catapult (MDC), characterised by functional assays, morphological analysis, and single-cell RNA sequencing. MDC then introduces a 3D CNS model created using Inventia Life Science’s RASTRUM™ bioprinter, illustrating how the cell model develops over time.
The presenters demonstrate how microglia are integrated into tri-culture systems and discuss the translation of imaging approaches when moving from 2D to 3D cultures. Key data include functional readouts of mitochondrial activity and cytokine responses following inflammatory stimulation.
The webinar concludes with a live Q&A on applying 3D human CNS models to drug discovery workflows.
Key learnings
- How 2D human microglia models are characterised using functional readouts, imaging and single-cell analysis
- What changes when transitioning from 2D to 3D CNS models
- How microglia integrate into 3D tri-culture systems
- How functional assays (mitochondrial activity, cytokine release) can be applied in 3D model systems
- What 3D human CNS systems can reveal about neuroinflammatory responses