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CRISPR-Ready ioGlutamatergic Neurons
User manual

CRISPR-Ready ioGlutamatergic Neurons

Human iPSC-derived glutamatergic neurons expressing Cas9 and dCas9 for CRISPR knockout, activation or interference.
CRISPR-Ready ioGlutamatergic Neurons
Human iPSC-derived glutamatergic neurons expressing Cas9 and dCas9 for CRISPR knockout, activation or interference.

CRISPR-Ready ioGlutamatergic Neurons mature rapidly into functional glutamatergic neurons after revival in the recommended medium. These cells constitutively express Cas9 or dCas9 nucleases, allowing users to perform single-gene knockouts, gene activation or gene intereference, pooled or arrayed CRISPR screens.

This user manual contains detailed instructions to culture the following products:


The document provides full details for cell culture and guide RNA delivery by either lentivirus transduction or lipid-based transfection and includes:

  • Culture of CRISPR-Ready ioGlutamatergic Neurons portfolio
  • Lipid-based delivery of synthetic guide RNA, including a protocol optimised for 96-well plate format
  • Lentiviral delivery of guide RNA
  • Immunofluorescence readout
  • Flow cytometry readout

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