Identification of neuronal subtype-specific splice variants in iPSC-derived cell models of ALS and FTD

You will learn:

• We identified alternately spliced genes in the TDP-43 mutant ioGlutamatergic and ioMotor Neurons relative to the wild type cells

• A large fraction of the alternatively spliced genes has been previously associated with ALS and/or FTD

• The ioGlutamatergic Neurons and ioMotor Neurons carrying the TDP-43 M337V mutation have relative to their wild type neurons both unique and shared alternatively spliced genes

• iCLIP TDP-43 RNA binding data shows that a large proportion of the identified alternatively spliced genes are bonafide TDP-43 targets

• Overall, our data suggests that the TDP-43 mutant ioGlutamatergic and ioMotor Neurons can be used to model aspects of ALS and FTD