Stimulation for cytokine secretion in ioMicroglia

A step-by-step protocol for quantifying the inflammatory response in ioMicroglia and ioMicroglia ioDisease Model Cells.

The assay uses a combination of immunostimulants including LPS and IFNγ combined, and Amyloid-β 1-42 (Aβ42) to model an inflammatory state. The subsequent secretion of cytokines can then be measured using a variety of assays, providing a robust platform for investigating neuroinflammatory pathways and profiling cellular responses to various stimuli.

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