protocol

mRNA transfection of ioSensory Neurons

This protocol is recommended for lipid-based delivery of synthetic mRNA into ioSensory Neurons in 96-well plates.

The synthetic mRNA delivery protocol was adapted from the manufacturer’s original protocol (Lipofectamine™ Stem Transfection Reagent, ThermoFisher Scientific).

The most robust transfection outcomes with synthetic mRNAs were achieved when delivery was performed 24 hours after cell revival. Transfection efficiency was subsequently monitored by imaging for up to 17 days post-delivery.

For access, please fill out the form on the right.

This protocol is recommended for lipid-based delivery of synthetic mRNA into ioSensory Neurons in 96-well plates.

The synthetic mRNA delivery protocol was adapted from the manufacturer’s original protocol (Lipofectamine™ Stem Transfection Reagent, ThermoFisher Scientific).

The most robust transfection outcomes with synthetic mRNAs were achieved when delivery was performed 24 hours after cell revival. Transfection efficiency was subsequently monitored by imaging for up to 17 days post-delivery.

For access, please fill out the form on the right.

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