Cell detachment protocol for ioMicroglia

A step-by-step protocol for detaching ioMicroglia and ioMicroglia ioDisease Model Cells.

Like all primary human microglia cells, ioMicroglia are adherent cells that form attachments to their surrounding environment. For many applications including flow cytometry, single cell RNA sequencing, and re-seeding for co-culture, microglia need to be detached from the plate. However, doing so is a delicate process and to ensure ioMicroglia remain viable, it is essential to perform gentle detachment from the culture dish.

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