Culturing ioGABAergic Neurons in 96-well plates

This step-by-step protocol provides details on how to culture of ioGABAergic Neurons and ioGABAergic Neurons ioDisease Model Cells in 96-well plates, enabling consistent, high-quality neuronal cultures for applications like high-content imaging, phenotypic screening, and functional assays.

Adapted for scalable workflows, this protocol maintains neuronal viability and morphology while enabling the efficient use of cells and reagents. Its format is ideal for automated, high-throughput applications in drug discovery and disease modelling. A key feature is the weekend-free feeding schedule, which supports operational efficiency by maintaining healthy cultures for at least 34 days without compromising performance.

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