Compound treatment in ioOligodendrocyte-like cells

This step-by-step protocol is recommended for screening small molecules in ioOligodendrocyte-like cells.

• The assay is designed for image-based readouts to quantify markers of maturation, like myelin basic protein (MBP), and to assess cell proliferation. The streamlined workflow allows treatment to begin on day 1 post-thaw, with final analysis on day 8.

• To demonstrate this compound treatment protocol, this guide uses TASIN-1 as a positive control. TASIN-1, a small molecule inhibitor of cholesterol biosynthesis, is a known pro-myelinating agent and is therefore expected to increase the expression of MBP in ioOligodendrocyte-like cells in a dose-dependent manner.