mapt N279K het overlay product page final

cat no | io1009

ioGlutamatergic Neurons
MAPT N279K/WT

Human iPSC-derived FTD disease model

A rapidly maturing, consistent and scalable isogenic system to study frontotemporal dementia (FTD).

ioGlutamatergic Neurons MAPTN279K/WT are opti-ox™ precision reprogrammed glutamatergic neurons containing a genetically engineered heterozygous N279K mutation in the MAPT gene encoding the tau protein. 

Disease model cells with the MAPT mutation are also available with a homozygous (cat no. io1008) and a heterozygous (cat no. io1015) P301S mutation.

Coming Soon

Register your interest, and we will notify you as soon as the product is available.

Unsure where to start?

Get in touch and we'll guide the way!

Benchtop benefits

defined_0

Make True Comparisons

Pair the ioDisease Model Cells with the genetically matched wild-type ioGlutamatergic Neurons to directly investigate the impact of mutant tau protein on disease.

scalable_0

Scalable

Industrial scale quantities are available with industry-leading seeding densities, and at a price point that allows the cells to be used from research to high throughput screening.

quick_0

Quick

The disease model cells and isogenic control are experiment ready as early as 2 days post revival, and form structural neuronal networks at 11 days.

Product information

Starting material

Human iPSC line

Karyotype

Normal (46, XY)

Seeding compatibility

6, 12, 24, 48, 96 & 384 well plates

Shipping info

Dry ice

Donor

Caucasian adult male (skin fibroblast)

Vial size

Small: >1 x 106 viable cells
Large: >5 x 106 viable cells

Quality control

Sterility, protein expression (ICC), gene expression (RT-qPCR) and genotype validation (Sanger sequencing)

Product use

These cells are for research use only

Differentiation method

opti-ox cellular reprogramming

Recommended seeding density

30,000 cells/cm2

User storage

LN2 or -150°C

Format

Cryopreserved cells

Applications

FTD research
Drug discovery and development
Disease modelling
Electrophysiological assays (MEA)
Co-culture studies

Genetic modification

Heterozygous N279K missense mutation in the MAPT gene

Product resources

Modelling neurodevelopment | Investigating the impact of maternal immune activation on neurodevelopment using human stem cell models Webinar
Modelling neurodevelopment | Investigating the impact of maternal immune activation on neurodevelopment using human stem cell models

Dr Deepak Srivastava | King’s College London

Watch now
Partnering with Charles River to advance CNS drug discovery with ioGlutamatergic Neurons™ Video
Partnering with Charles River to advance CNS drug discovery with ioGlutamatergic Neurons™

Charles River

Watch now
How to culture ioGlutamatergic Neurons™ Video tutorial
How to culture ioGlutamatergic Neurons™

Dr Kaiser Karim | Scientist
bit.bio

Watch now
How to prepare tissue culture vessels with PDL-Geltrex™ for ioGlutamatergic Neuron™ culture Video tutorial
How to prepare tissue culture vessels with PDL-Geltrex™ for ioGlutamatergic Neuron™ culture

Dr Kaiser Karim | Scientist
bit.bio

Watch now
In Conversation with Charles River Video
In Conversation with Charles River

Dr Marijn Vlaming | Head of Biology
Charles River

Watch now
High Content Analysis of 2D and 3D cellular models for target and phenotypic drug discovery Poster
High Content Analysis of 2D and 3D cellular models for target and phenotypic drug discovery

Lachize | et al
Courtesy of Charles River Laboratories

Download
ioGlutamatergic Neurons™ Brochure
ioGlutamatergic Neurons™

bit.bio

Download

Addressing current challenges of in vitro cell models 

Read this blog to find out how experts from across academia and industry are approaching the challenges of reproducibility of in vitro cell models as well as potential solutions.

bit-bio_Addressing the challenges of cell models_Blog_Image1200x755

Related products

ioGlutamatergic Neurons MAPT P301S/WT™ ioDisease Model
ioGlutamatergic Neurons MAPT P301S/WT™ cat no. io1015
Order now
ioGlutamatergic Neurons MAPT P301S/P301S™ ioDisease Model
ioGlutamatergic Neurons MAPT P301S/P301S™ cat no. io1018
Order now
ioMicroglia™ ioWild Type
ioMicroglia™ cat no. ioA021
Order now
ioGlutamatergic Neurons TDP-43 M337V™ ioDisease Model
ioGlutamatergic Neurons TDP-43 M337V™ cat no. ioEA1005 | ioEA1006
Order now
ioGABAergic Neurons™ ioWild Type
ioGABAergic Neurons™ cat no. ioEA1003S
Order now
ioSkeletal Myocytes™ ioWild Type
ioSkeletal Myocytes™ cat no. io1002
Order now
ioGlutamatergic Neurons HTT50CAG/WT™ ioDisease Model
ioGlutamatergic Neurons HTT50CAG/WT™ cat no. ioEA1004
Order now
ioGlutamatergic Neurons™ ioWild Type
ioGlutamatergic Neurons™ cat no. io1001
Order now

Wild Type and Isogenic Disease Model cells: A true comparison.

Further your disease research by pairing our wild type cells with isogenic disease models.

bitbio-vials-Wild_and_Disease-staggered-2500px_wide

Related pages

Discover ioCells Learn about our range of human iPSC-derived cells for research and drug discovery
Resources Explore our latest scientific insights, webinars, blogs and videos
Our platform Discover the cell identity coding platform that powers our ioCells