Poster

Rapid and consistent generation of functional motor neurons from reprogrammed human iPSCs using opti-ox technology

This poster was presented by bit.bio at Society for Neuroscience 2023 international conference, demonstrating the use of opti-ox technology to rapidly reprogram hiPSCs into motor neurons, termed ioMotor Neurons.
Rapid and consistent generation of functional motor neurons from reprogrammed human iPSCs using opti-ox technology
This poster was presented by bit.bio at Society for Neuroscience 2023 international conference, demonstrating the use of opti-ox technology to rapidly reprogram hiPSCs into motor neurons, termed ioMotor Neurons.
We have used opti-ox technology to rapidly reprogram hiPSCs into motor neurons, termed ioMotor Neurons, which are a homogenous population of cells with classical neuronal morphology and neurite outgrowth. As early as 4 days in culture, cells express the pan-neuronal markers MAP2 and TUBB3, the cholinergic markers ChAT and VAChT and the motor neuron-specific markers MNX1 and ISL1/2, as assessed by both ICC and RT-qPCR. Bulk RNA sequencing of ioMotor Neurons demonstrates a rapid acquisition of a motor neuron signature. ioMotor Neurons show spontaneous neuronal activity with increasing firing rate over 40 days in culture, as shown by multielectrode array activity (MEA).

Vaquero, et al

bit.bio

2023

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