A novel human skeletal muscle in vitro model using opti-ox mediated cellular reprogramming of induced pluripotent stem cells
![A novel human skeletal muscle in vitro model using opti-ox mediated cellular reprogramming of induced pluripotent stem cells](https://www.bit.bio/hs-fs/hubfs/Website%20content/Product%20pages/ioSkeletal%20Myocytes/ioSkeletal-Myocytes-2550px_wide-banner-comp.jpeg?width=2550&height=1913&name=ioSkeletal-Myocytes-2550px_wide-banner-comp.jpeg)
Through targeting of genomic safe harbour loci, we used opti-ox to achieve homogenous, inducible expression of key transcription factors. Transcription factor induction leads to shutdown of the core pluripotency network, and activation of key myogenic factors including myosin heavy chain.
opti-ox reprogrammed ioSkeletal Myocytes express Desmin, Dystrophin and Titin, and form contractile, striated and multinucleated myocytes by Day 10 post-revival. Critically for metabolic studies, robust expression of the insulin-regulated glucose transporter GLUT4 is also detected. Importantly, skeletal myocytes produce a highly pure MHC positive population of cells within 4 days of thawing, comparable to day 10 transdifferentiated fibroblasts, and are suitable for phenotypic-based HTS.