Stimulating 3D Skeletal Muscle Microtissues in a Novel Perfusable Microphysiological System with Integrated Electrodes

Bi/ond have developed the MUSbit™, a novel microphysiological system with a microfluidic channel for perfusion, and pillars designed for anchoring muscle cell bundles. bit.bio’s human iPSC-derived skeletal muscle cells were encapsulated within a 3D extracellular matrix hydrogel on the MUSbit™ chip and cultured over 14 days, resulting in 3D microtissues that expressed muscle cell markers and contracted in response to electrical stimulation.

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• Successful 3D muscle bundle formation on the MUSbit™ microchip
• Mature cross-striated muscle bundles expressing muscle cell markers
• Robust twitch and tetanus contraction following electrical stimulation
• Features of the MUSbit™ microchip and the prototype Let-it-bit™ microchip with functional integrated electrodes

The data demonstrates the successful generation of engineered 3D skeletal muscle microtissues using bit.bio’s ioSkeletal Mycoytes on the Bi/ond microchips, which have the capability for perfusion of muscle tissues and integration of functional electrodes. The bit.bio cells and Bi/ond microphysiological systems combine to offer a system for measuring muscle tissue contractile performance and investigating pharmacological interventions in wild-type and disease model cells, such as bit.bio’s human iPSC-derived Duchenne muscular dystrophy models with a DMD exon 44 deletion or DMD exon 52 deletion.

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