Poster

Rapid and consistent generation of functional motor neurons from reprogrammed human iPSCs using opti-ox™ technology

This poster was presented by bit.bio at Society for Neuroscience 2023 international conference, demonstrating the use of opti-ox technology to rapidly reprogram hiPSCs into motor neurons, termed ioMotor Neurons™.
Rapid and consistent generation of functional motor neurons from reprogrammed human iPSCs using opti-ox™ technology
This poster was presented by bit.bio at Society for Neuroscience 2023 international conference, demonstrating the use of opti-ox technology to rapidly reprogram hiPSCs into motor neurons, termed ioMotor Neurons™.
We have used opti-ox technology to rapidly reprogram hiPSCs into motor neurons, termed ioMotor Neurons™, which are a homogenous population of cells with classical neuronal morphology and neurite outgrowth. As early as 4 days in culture, cells express the pan-neuronal markers MAP2 and TUBB3, the cholinergic markers ChAT and VAChT and the motor neuron-specific markers MNX1 and ISL1/2, as assessed by both ICC and RT-qPCR. Bulk RNA sequencing of ioMotor Neurons demonstrates a rapid acquisition of a motor neuron signature. ioMotor Neurons show spontaneous neuronal activity with increasing firing rate over 40 days in culture, as shown by multielectrode array activity (MEA). 

Vaquero, et al

bit.bio

2023

Related pages

News Read our latest updates and press coverage
Our platform Discover the cell identity coding platform behind our cells
Join us Explore the latest roles at bit.bio