Human iPSC-derived GABAergic neurons
ioRD003X | In development
ioGABAergic Neurons have been reprogrammed from human induced pluripotent stem cells (iPSCs) using our precise reprogramming technology: opti-ox™1 (optimised inducible overexpression). Human stem cells, within days, convert into consistent, functional inhibitory GABAergic neurons providing a high-quality human model for the study of neurological connectivity and disorders.
ioGABAergic Neurons, part of our ioCells portfolio, consist of GABAergic neurons (>95%) characterised by the expression of the classical marker genes including GAD1, GAD2, VGAT, DLX1 and DLX2. Single cell RNA sequencing analysis shows that ioGABAergic Neurons have characteristics of the vasoactive intestinal polypeptide (VIP) subtype of GABAergic neurons and rapidly form neuronal networks with spontaneous neuronal activity by day 20 post-revivial.
Ready-to-culture cells are suitable as models for research in cell-type specific biology, target validation and drug screening in pharmaceutical R&D.
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ioGABAergic Neurons show mature neuronal networks by Day 11
ioGABAergic Neurons show characteristics of the vasoactive intestinal polypeptide (VIP) subtype
Bioinformatic analysis of GABAergic subtype identity from single cell RNA-seq data. Anchoring our single cell RNA-seq data of the iPSC-derived GABAergic neurons on the Allen Brain single cell map of the human cortex demonstrates that the ioGABAergic Neurons (red cluster) closely match with the VIP subtype of GABAergic neurons (purple cluster).
ioGABAergic Neurons are delivered in a cryopreserved format and are programmed to rapidly mature upon revival in the recommended media. The protocol for the generation of these cells is a three-phase process: Induction, which is carried out at bit.bio (Phase 0), Stabilisation for 3 days with Doxycycline (Phase 1), and Maintenance (Phase 2) during which the ioGABAergic Neurons mature. Phases 1 and 2 after revival of cells are carried out at the customer site.
human iPSC line
caucasian adult male
opti-oxTM cellular reprogramming
Recommended seeding density
Sterility, protein expression (IF) and gene expression (RT-qPCR)
LN2 or -150°C
These cells are for research use only