Scalable and well defined human glutamatergic and gabaergic co-culture platform for studying excitatory-inhibitory neuron imbalances and the discovery of drugs to treat associated diseases

You will learn:

• Molecular characterisation shows that the ioGABAergic Neurons are a highly pure population (>99%) of neurons expressing key GABAergic marker genes.

• The MEA data demonstrates that ioGlutamatergic Neurons and ioGABAergic Neurons consist of only excitatory and inhibitory neurons respectively, which is in line with the transcriptomics data of both cell types.

• ioGABAergic Neurons function as true inhibitory neurons, and drugs interfering with GABAergic signaling suggest that these neurons release GABA, thereby inhibiting the activity of the excitatory ioGlutamatergic Neurons.

• ioGABAergic Neurons and ioGlutamatergic Neurons form functional circuits.

In summary, opti-ox deterministic cell programming enables the manufacturing of highly pure (>99%), consistent, and functional GABAergic neurons that can serve as a high-quality human model to study both neurodevelopment and neurological disorders, including epilepsy.