CNS co-culture
Co-culture of hiPSC-derived ioGlutamatergic Neurons and ioMicroglia
Protocol for culturing human iPSC-derived glutamatergic neurons (ioGlutamatergic Neurons) and microglia cells (ioMicroglia).
CNS tri-culture
Tri-culture of ioGlutamatergic Neurons, ioGABAergic Neurons and astrocytes in 48-well plates for functional MEA analysis
Protocol to tri-culture ioGlutamatergic Neurons, ioGABAergic Neurons and astrocytes in 48-well plates for functional micro-electrode array (MEA) analysis using Axion Maestro Pro
Cell culture and preparation
Cell detachment for flow cytometry and single cell experiments
Protocol for detaching human iPSC-derived microglia cells for flow cytometry and other single cell experiments.
Cell characterisation and assays
Immunocytochemical staining protocol for human iPSC-derived GABAergic neurons
Immunocytochemical staining of human iPSC-derived GABAergic neurons to identify, characterise, and validate in vitro.
Immunocytochemical staining protocol for human iPSC-derived glutamatergic neurons
Immunocytochemical staining of human iPSC-derived glutamatergic neurons to identify, characterise, and validate in vitro.
ioMicroglia stimulation for cytokine release
Protocol describing the functional characterisation of human iPSC-derived microglia cells via stimulation and cytokine release.
Immunocytochemistry staining protocol for human iPSC-derived oligodendrocyte-like cells
Immunocytochemistry staining of human iPSC-derived oligodendrocyte-like cells with O4 and MBP antibodies.
Immunocytochemical staining protocol for human iPSC-derived skeletal myocytes
Immunocytochemical staining of human iPSC-derived skeletal myocytes to identify, characterise, and validate in vitro.