Optimised and scalable programming of human iPSCs to generate nociceptor sensory neurons for the study of pain mechanisms and neuropathies

You will learn:

• ioSensory Neurons have a defined nociceptor identity as shown by the expression of key nociceptor marker genes, NTRK1 and TRP ion channels, including TRPV1. These cells display spontaneous activity by MEA (data not shown) and display a functional nociceptor phenotype, as demonstrated by responsiveness to heat and selective agonists for TRPM3, and TRPM8.

• ioSensory neurons demonstrate expression of the functional TTX resistant sodium channels, characteristic of nociceptors, and respond to NaV1.8 blockers.

• ioSensory Neurons mature rapidly and are easy to culture using a simple 1- medium, 2-step mitomycin C-free protocol – ideal for researchers without iPSC expertise.

• ioSensory neurons show batch-to batch reproducibility and homogeneity, as shown by bulk RNA sequencing.